In Vivo Imaging of Local Gene Expression Induced by Magnetic Hyperthermia
| hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
| hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
| dc.contributor.author | SANDRE, Olivier | |
| hal.structure.identifier | Imagerie moléculaire et thérapies innovantes en oncologie [IMOTION] | |
| dc.contributor.author | GENEVOIS, Coralie | |
| hal.structure.identifier | Universidad del País Vasco [Espainia] / Euskal Herriko Unibertsitatea [España] = University of the Basque Country [Spain] = Université du pays basque [Espagne] [UPV / EHU] | |
| dc.contributor.author | GARAIO, Eneko | |
| hal.structure.identifier | Institut de Chimie de la Matière Condensée de Bordeaux [ICMCB] | |
| dc.contributor.author | ADUMEAU, Laurent | |
| hal.structure.identifier | Institut de Chimie de la Matière Condensée de Bordeaux [ICMCB] | |
| dc.contributor.author | MORNET, Stéphane | |
| hal.structure.identifier | Imagerie moléculaire et thérapies innovantes en oncologie [IMOTION] | |
| dc.contributor.author | COUILLAUD, Franck | |
| dc.date.issued | 2017 | |
| dc.identifier.issn | 2073-4425 | |
| dc.description.abstractEn | The present work aims to demonstrate that colloidal dispersions of magnetic iron oxide nanoparticles stabilized with dextran macromolecules placed in an alternating magnetic field can not only produce heat, but also that these particles could be used in vivo for local and noninvasive deposition of a thermal dose sufficient to trigger thermo-induced gene expression. Iron oxide nanoparticles were first characterized in vitro on a bio-inspired setup, and then they were assayed in vivo using a transgenic mouse strain expressing the luciferase reporter gene under transcriptional control of a thermosensitive promoter. Iron oxide nanoparticles dispersions were applied topically on the mouse skin or injected subcutaneously with Matrigel™ to generate so-called pseudotumors. Temperature was monitored continuously with a feedback loop to control the power of the magnetic field generator and to avoid overheating. Thermo-induced luciferase expression was followed by bioluminescence imaging 6 h after heating. We showed that dextran-coated magnetic iron oxide nanoparticle dispersions were able to induce in vivo mild hyperthermia compatible with thermo-induced gene expression in surrounding tissues and without impairing cell viability. These data open new therapeutic perspectives for using mild magnetic hyperthermia as noninvasive modulation of tumor microenvironment by local thermo-induced gene expression or drug release. | |
| dc.description.sponsorship | Magnéto-Chimiothérapie : Modélisation de la Délivrance Induite par Champ Magnétique Radiofréquence d'Anticancéreux par des Nano-Vésicules Polymères et Suivi par IRM d'un Modèle de Glioblastome - ANR-13-BS08-0017 | |
| dc.description.sponsorship | Initiative d'excellence de l'Université de Bordeaux - ANR-10-IDEX-0003 | |
| dc.language.iso | en | |
| dc.publisher | MDPI | |
| dc.rights.uri | http://creativecommons.org/licenses/by/ | |
| dc.subject.en | magnetic polymer-coated nanoparticles | |
| dc.subject.en | in vivo optical imaging | |
| dc.subject.en | heat shock protein promoter | |
| dc.subject.en | gene therapies | |
| dc.subject.en | magnetic hyperthermia | |
| dc.title.en | In Vivo Imaging of Local Gene Expression Induced by Magnetic Hyperthermia | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.3390/genes8020061 | |
| dc.subject.hal | Chimie/Matériaux | |
| dc.subject.hal | Chimie/Polymères | |
| dc.subject.hal | Physique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft] | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Ingénierie biomédicale | |
| dc.description.sponsorshipEurope | Multifunctional Nanoparticles for Magnetic Hyperthermia and Indirect Radiation Therapy | |
| bordeaux.journal | Genes | |
| bordeaux.page | 61 | |
| bordeaux.volume | 8 | |
| bordeaux.issue | 2 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-01462355 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-01462355v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Genes&rft.date=2017&rft.volume=8&rft.issue=2&rft.spage=61&rft.epage=61&rft.eissn=2073-4425&rft.issn=2073-4425&rft.au=SANDRE,%20Olivier&GENEVOIS,%20Coralie&GARAIO,%20Eneko&ADUMEAU,%20Laurent&MORNET,%20St%C3%A9phane&rft.genre=article |
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