High Temperature and High Hydrostatic Pressure Cultivation, Transfer, and Filtration Systems for Investigating Deep Marine Microorganisms
| hal.structure.identifier | Department of Earth and Environmental Sciences [Troy, NY] | |
| dc.contributor.author | OLIVER, Gina Carole | |
| hal.structure.identifier | Department of Earth and Environmental Sciences [Troy, NY] | |
| dc.contributor.author | CARIO, Anaïs | |
| hal.structure.identifier | Department of Earth and Environmental Sciences [Troy, NY] | |
| hal.structure.identifier | Rensselaer Astrobiology Research and Education Center | |
| dc.contributor.author | ROGERS, Karyn Lynne | |
| dc.date.accessioned | 2022-10-12T08:52:44Z | |
| dc.date.available | 2022-10-12T08:52:44Z | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/150300 | |
| dc.description.abstractEn | High temperatures (HT) and high hydrostatic pressures (HHP) are characteristic of deep-sea hydrothermal vents and other deep crustal settings. These environments host vast and diverse microbial populations, yet only a small fraction of those populations have been successfully cultured. This is due, in part, to the difficulty of sampling while maintaining these in situ conditions and also replicating those high-temperature and high-pressure conditions in the laboratory. In an effort to facilitate more HT-HHP cultivation, we present two HT-HHP batch culture incubation systems for cultivating deep-sea vent and subsurface (hyper)thermophilic microorganisms. One HT-HHP system can be used for batch cultivation up to 110 MPa and 121°C, and requires sample decompression during subsampling. The second HT-HHP system can be used to culture microorganisms up to 100 MPa and 160°C with variable-volume, pressure-retaining vessels that negate whole-sample decompression during subsampling. Here, we describe how to build cost effective heating systems for these two types of high-pressure vessels, as well as the protocols for HT-HHP microbial batch cultivation in both systems. Additionally, we demonstrate HHP transfer between the variable-volume vessels, which has utility in sampling and enrichment without decompression, laboratory isolation experiments, as well as HHP filtration. | |
| dc.language.iso | en | |
| dc.title.en | High Temperature and High Hydrostatic Pressure Cultivation, Transfer, and Filtration Systems for Investigating Deep Marine Microorganisms | |
| dc.type | Document de travail - Pré-publication | |
| dc.identifier.doi | 10.20944/preprints202104.0453.v1 | |
| dc.subject.hal | Chimie/Matériaux | |
| bordeaux.hal.laboratories | Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB) - UMR 5026 | * |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.institution | Bordeaux INP | |
| bordeaux.institution | CNRS | |
| hal.identifier | hal-03338547 | |
| hal.version | 1 | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-03338547v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=OLIVER,%20Gina%20Carole&CARIO,%20Ana%C3%AFs&ROGERS,%20Karyn%20Lynne&rft.genre=preprint |
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