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Cryo-electron tomography of nanoparticle transmigration into liposome.

hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLE BIHAN, Olivier
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBONNAFOUS, Pierre
hal.structure.identifierElectronique, Systèmes de communication et Microsystèmes [ESYCOM]
dc.contributor.authorMARAK, Laszlo
hal.structure.identifierCentre de physique moléculaire optique et hertzienne [CPMOH]
dc.contributor.authorBICKEL, Thomas
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorTRÉPOUT, Sylvain
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorMORNET, Stéphane
hal.structure.identifierFEI Electron Optics
dc.contributor.authorDE HAAS, Felix
hal.structure.identifierLaboratoire Algorithmique et Architecture des Systèmes Informatiques [A2SI]
dc.contributor.authorTALBOT, Hugues
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorTAVEAU, Jean-Christophe
hal.structure.identifierImagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie [IECB]
dc.contributor.authorLAMBERT, Olivier
dc.date.issued2009
dc.identifier.issn1047-8477
dc.description.abstractEnNanoparticle transport across cell membrane plays a crucial role in the development of drug delivery systems as well as in the toxicity response induced by nanoparticles. As hydrophilic nanoparticles interact with lipid membranes and are able to induce membrane perturbations, hypothetic mechanisms based on membrane curvature or hole formation have been proposed for activating their transmigration. We report on the transport of hydrophilic silica nanoparticles into large unilamellar neutral DOPC liposomes via an internalization process. The strong adhesive interactions of lipid membrane onto the silica nanoparticle triggered liposome deformation until the formation of a curved neck. Then the rupture of this membrane neck led to the complete engulfment of the nanoparticle. Using cryo-electron tomography we determined 3D architectures of intermediate steps of this process unveiling internalized silica nanoparticles surrounded by a supported lipid bilayer. This engulfing process was achieved for a large range of particle size (from 30 to 200 nm in diameter). These original data provide interesting highlights for nanoparticle transmigration and could be applied to biotechnology development.
dc.language.isoen
dc.publisherElsevier
dc.title.enCryo-electron tomography of nanoparticle transmigration into liposome.
dc.typeArticle de revue
dc.identifier.doi10.1016/j.jsb.2009.07.006
dc.subject.halChimie/Matériaux
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
dc.subject.halInformatique [cs]/Biotechnologie
bordeaux.journalJournal of Structural Biology
bordeaux.page419-25
bordeaux.volume168
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-00436243
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00436243v1
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