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The effect of surface energy, adsorbed RGD peptides and fibronectin on the attachment and spreading of cells on multiwalled carbon nanotube papers

hal.structure.identifierCentre de recherches Paul Pascal [CRPP]
dc.contributor.authorVIDAL, Guillaume
hal.structure.identifierCentre de recherches Paul Pascal [CRPP]
dc.contributor.authorDELORD, Brigitte
hal.structure.identifierCentre de recherches Paul Pascal [CRPP]
dc.contributor.authorNERI, Wilfrid
hal.structure.identifierCentre de recherches Paul Pascal [CRPP]
dc.contributor.authorGOUNEL, Sébastien
hal.structure.identifierCentre de recherches Paul Pascal [CRPP]
dc.contributor.authorBARTHOLOME, Christèle
hal.structure.identifierCentre de recherches Paul Pascal [CRPP]
dc.contributor.authorLY, Isabelle
hal.structure.identifierCentre de recherches Paul Pascal [CRPP]
dc.contributor.authorPOULIN, Philippe
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorLABRUGÈRE, Christine
hal.structure.identifierCentre de Ressources en Microscopie Electronique et Microanalyse
dc.contributor.authorSELLIER, Elisabeth
hal.structure.identifierBiomateriaux et Reparation Tissulaire
dc.contributor.authorDURRIEU, Marie-Christine
hal.structure.identifierBiomateriaux et Reparation Tissulaire
dc.contributor.authorAMÉDÉE, Joëlle
dc.date.issued2011
dc.identifier.issn0008-6223
dc.description.abstractEnWe report that hydrophilic oxidized multiwalled carbon nanotube papers (CNPs) were efficient substrates for attachment, spreading, and proliferation of MG-63 cells. Complete spreading occurred in the first 4 h of culture even without complete serum or pre-adsorbed adhesion proteins such as collagen or fibronectin. By contrast, the density of adherent cell on hydrophobic CNPs was low. Spreading did not happen after 24 h of culture in absence of serum proteins and growth factors on such papers. Cell viability concomitantly decreased. We also observed that a short RGD peptide, designed to adsorb on both hydrophobic and hydrophilic CNPs, enhanced adhesion, spreading, and proliferation only on hydrophilic CNPs. By contrast, adsorbed fibronectin triggers cell adhesion and spreading on both types of CNPs. Our results show that the imitation of tissue basal membranes with the nanofibrous structure of CNPs is insufficient to trigger the proliferation and spread of cells. Chemical cues are also needed for cells to spread. CNPs, however, offer a better substrate for adhesion protein absorption than do non-porous polymers.
dc.language.isoen
dc.publisherElsevier
dc.title.enThe effect of surface energy, adsorbed RGD peptides and fibronectin on the attachment and spreading of cells on multiwalled carbon nanotube papers
dc.typeArticle de revue
dc.identifier.doi10.1016/j.carbon.2011.01.064
dc.subject.halChimie/Matériaux
bordeaux.journalCarbon
bordeaux.page2318-2333
bordeaux.volume49
bordeaux.issue7
bordeaux.peerReviewedoui
hal.identifierhal-00589663
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00589663v1
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