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Magnetic resonance imaging tracking of human adipose derived stromal cells within three-dimensional scaffolds for bone tissue engineering.

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorLALANDE, C.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorMIRAUX, Sylvain
hal.structure.identifierHémostase, bio-ingénierie et remodelage cardiovasculaires [LBPC (U698)]
dc.contributor.authorDERKAOUI, Mohammed
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorMORNET, Stéphane
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBAREILLE, Reine
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFRICAIN, Jean-Christophe
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorFRANCONI, Jean-Michel
hal.structure.identifierHémostase, bio-ingénierie et remodelage cardiovasculaires [LBPC (U698)]
dc.contributor.authorLE VISAGE, Catherine
hal.structure.identifierHémostase, bio-ingénierie et remodelage cardiovasculaires [LBPC (U698)]
dc.contributor.authorLETOURNEUR, Didier
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorAMÉDÉE, Joëlle Vilamitjana
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorBOUZIER-SORE, Anne-Karine
dc.date.issued2011
dc.identifier.issn1473-2262
dc.description.abstractEnFor bone tissue engineering, human Adipose Derived Stem Cells (hADSCs) are proposed to be associated with a scaffold for promoting bone regeneration. After implantation, cellularised scaffolds require a non-invasive method for monitoring their fate in vivo. The purpose of this study was to use Magnetic Resonance Imaging (MRI)-based tracking of these cells, labelled with magnetic agents for in vivo longitudinal assessment. hADSCs were isolated from adipose tissue and labelled with USPIO-rhodamine (Ultrasmall SuperParamagnetic Iron Oxide). USPIO internalisation, absence of toxicity towards hADSCs, and osteogenic differentiation of the labelled cells were evaluated in standard culture conditions. Labelled cells were then seeded within a 3D porous polysaccharide-based scaffold and imaged in vitro using fluorescence microscopy and MRI. Cellularised scaffolds were implanted subcutaneously in nude mice and MRI analyses were performed from 1 to 28 d after implantation. In vitro, no effect of USPIO labelling on cell viability and osteogenic differentiation was found. USPIO were efficiently internalised by hADSCs and generated a high T2* contrast. In vivo MRI revealed that hADSCs remain detectable until 28 d after implantation and could migrate from the scaffold and colonise the area around it. These data suggested that this scaffold might behave as a cell carrier capable of both holding a cell fraction and delivering cells to the site of implantation. In addition, the present findings evidenced that MRI is a reliable technique to validate cell-seeding procedures in 3D porous scaffolds, and to assess the fate of hADSCs transplanted in vivo.
dc.language.isoen
dc.publisherAO Research Institute Davos
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/
dc.title.enMagnetic resonance imaging tracking of human adipose derived stromal cells within three-dimensional scaffolds for bone tissue engineering.
dc.typeArticle de revue
dc.identifier.doi10.22203/eCM.v021a25
dc.subject.halChimie/Matériaux
bordeaux.journaleCells and Materials Journal
bordeaux.page341-354
bordeaux.volume21
bordeaux.peerReviewedoui
hal.identifierhal-00594106
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00594106v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=eCells%20and%20Materials%20Journal&rft.date=2011&rft.volume=21&rft.spage=341-354&rft.epage=341-354&rft.eissn=1473-2262&rft.issn=1473-2262&rft.au=LALANDE,%20C.&MIRAUX,%20Sylvain&DERKAOUI,%20Mohammed&MORNET,%20St%C3%A9phane&BAREILLE,%20Reine&rft.genre=article


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