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Cytotoxicity and oxidative stress induced by different metallic nanoparticles on human kidney cells.

hal.structure.identifierLaboratoire de Biologie cellulaire
dc.contributor.authorPUJALTÉ, Igor
hal.structure.identifierLaboratoire de Biologie cellulaire
dc.contributor.authorPASSAGNE, Isabelle
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBROUILLAUD, Brigitte
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorTRÉGUER-DELAPIERRE, Mona
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorDURAND, Etienne
hal.structure.identifierLaboratoire Hydrologie-Environnement
dc.contributor.authorOHAYON-COURTÈS, Céline
hal.structure.identifierLaboratoire de Biologie cellulaire
dc.contributor.authorL'AZOU, Béatrice
dc.date.issued2011
dc.identifier.issn1743-8977
dc.description.abstractEnBACKGROUND: Some manufactured nanoparticles are metal-based and have a wide variety of applications in electronic, engineering and medicine. Until now, many studies have described the potential toxicity of NPs on pulmonary target, while little attention has been paid to kidney which is considered to be a secondary target organ. The objective of this study, on human renal culture cells, was to assess the toxicity profile of metallic nanoparticles (TiO2, ZnO and CdS) usable in industrial production. Comparative studies were conducted, to identify whether particle properties impact cytotoxicity by altering the intracellular oxidative status. RESULTS: Nanoparticles were first characterized by size, surface charge, dispersion and solubility. Cytotoxicity of NPs was then evaluated in IP15 (glomerular mesangial) and HK-2 (epithelial proximal) cell lines. ZnO and CdS NPs significantly increased the cell mortality, in a dose-dependent manner. Cytotoxic effects were correlated with the physicochemical properties of NPs tested and the cell type used. Analysis of reactive oxygen species and intracellular levels of reduced and oxidized glutathione revealed that particles induced stress according to their composition, size and solubility. Protein involved in oxidative stress such as NF-κb was activated with ZnO and CdS nanoparticles. Such effects were not observed with TiO2 nanoparticles. CONCLUSION: On glomerular and tubular human renal cells, ZnO and CdS nanoparticles exerted cytotoxic effects that were correlated with metal composition, particle scale and metal solubility. ROS production and oxidative stress induction clearly indicated their nephrotoxic potential.
dc.language.isoen
dc.publisherBioMed Central
dc.subject.meshCadmium Compounds
dc.subject.meshCell Line
dc.subject.meshSulfides
dc.subject.meshTitanium
dc.subject.meshToxicity Tests
dc.subject.meshZinc Oxide
dc.subject.meshCell Survival
dc.subject.meshHumans
dc.subject.meshKidney
dc.subject.meshMetal Nanoparticles
dc.subject.meshNF-kappa B
dc.subject.meshOxidative Stress
dc.subject.meshParticle Size
dc.subject.meshReactive Oxygen Species
dc.title.enCytotoxicity and oxidative stress induced by different metallic nanoparticles on human kidney cells.
dc.typeArticle de revue
dc.identifier.doi10.1186/1743-8977-8-10
dc.subject.halSciences du Vivant [q-bio]/Santé publique et épidémiologie
dc.subject.halSciences du Vivant [q-bio]/Toxicologie
bordeaux.journalParticle and Fibre Toxicology
bordeaux.page10
bordeaux.volume8
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierinserm-00617214
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-00617214v1
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