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In vivo MR tracking of therapeutic microglia to a human glioma model.

hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorRIBOT, Emeline J.
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorMIRAUX, Sylvain
hal.structure.identifierUnité de Psychoneuroimmunologie, Nutrition et Génétique [PsyNuGen]
dc.contributor.authorKONSMAN, Jan P.
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorBOUCHAUD, Véronique
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorPOURTAU, Line
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorDELVILLE, Marie-Hélène
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorFRANCONI, Jean-Michel
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorTHIAUDIÈRE, Eric
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorVOISIN, Pierre J.
dc.date.issued2011
dc.identifier.issn0952-3480
dc.description.abstractEnA knowledge of the spatial localization of cell vehicles used in gene therapy against glioma is necessary before launching therapy. For this purpose, MRI cell tracking is performed by labeling the cell vehicles with contrast agents. In this context, the goal of this study was to follow noninvasively the chemoattraction of therapeutic microglial cells to a human glioma model before triggering therapy. Silica nanoparticles grafted with gadolinium were used to label microglia. These vehicles, expressing constitutively the thymidine kinase suicide gene fused to the green fluorescent protein gene, were injected intravenously into human glioma-bearing nude mice. MRI was performed at 4.7 T to track noninvasively microglial accumulation in the tumor. This was followed by microscopy on brain slices to assess the presence in the glioma of the contrast agents, microglia and fusion gene through the detection of silica nanoparticles grafted with tetramethyl rhodamine iso-thiocyanate, 3,3'-dioctadecyloxacarbocyanine perchlorate and green fluorescent protein fluorescence, respectively. Finally, gancyclovir was administered systemically to mice. Human microglia were detectable in living mice, with strong negative contrast on T(2) *-weighted MR images, at the periphery of the glioma only 24 h after systemic injection. The location of the dark dots was identical in MR microscopy images of the extracted brains at 9.4 T. Fluorescence microscopy confirmed the presence of the contrast agents, exogenous microglia and suicide gene in the intracranial tumor. In addition, gancyclovir treatment allowed an increase in mice survival time. This study validates the MR tracking of microglia to a glioma after systemic injection and their use in a therapeutic strategy against glioma. Copyright © 2011 John Wiley & Sons, Ltd.
dc.language.isoen
dc.publisherWiley
dc.title.enIn vivo MR tracking of therapeutic microglia to a human glioma model.
dc.typeArticle de revue
dc.identifier.doi10.1002/nbm.1699
dc.subject.halChimie/Matériaux
bordeaux.journalNMR in Biomedicine
bordeaux.page1361-1368
bordeaux.volume24
bordeaux.issue10
bordeaux.peerReviewedoui
hal.identifierhal-00657909
hal.version1
hal.popularnon
hal.audienceInternationale
dc.subject.itMRI
dc.subject.itCell tracking
dc.subject.itMicroglia
dc.subject.itGlioma
dc.subject.itGene therapy
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00657909v1
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