Lysophosphatidic acid acyltransferases: a link with intracellular protein trafficking in Arabidopsis root cells?
| dc.rights.license | open | en_US |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | WATTELET-BOYER, Valerie | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | LE GUEDARD, Marina | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | DITTRICH-DOMERGUE, Franziska | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | MANETA-PEYRET, Lilly | |
| dc.contributor.author | KRIECHBAUMER, Verena | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | BOUTTE, Yohann | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | BESSOULE, Jean-Jacques
ORCID: 0000-0002-5717-5210 IDREF: 072151846 | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | MOREAU, Patrick
IDREF: 058610723 | |
| dc.date.accessioned | 2022-10-04T09:01:54Z | |
| dc.date.available | 2022-10-04T09:01:54Z | |
| dc.date.issued | 2022-03-02 | |
| dc.identifier.issn | 1460-2431 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/148345 | |
| dc.description.abstractEn | Phosphatidic acid (PA) and lysophosphatidic acid acyltransferases (LPAATs) might be critical for the secretory pathway. Four extra-plastidial LPAATs (LPAAT2, 3, 4, and 5) were identified in Arabidopsis thaliana. These AtLPAATs display a specific enzymatic activity converting lysophosphatidic acid to PA and are located in the endomembrane system. We investigate a putative role for AtLPAATs 3, 4, and 5 in the secretory pathway of root cells through genetical (knockout mutants), biochemical (activity inhibitor, lipid analyses), and imaging (live and immuno-confocal microscopy) approaches. Treating a lpaat4;lpaat5 double mutant with the LPAAT inhibitor CI976 produced a significant decrease in primary root growth. The trafficking of the auxin transporter PIN2 was disturbed in this lpaat4;lpaat5 double mutant treated with CI976, whereas trafficking of H+-ATPases was unaffected. The lpaat4;lpaat5 double mutant is sensitive to salt stress, and the trafficking of the aquaporin PIP2;7 to the plasma membrane in the lpaat4;lpaat5 double mutant treated with CI976 was reduced. We measured the amounts of neo-synthesized PA in roots, and found a decrease in PA only in the lpaat4;lpaat5 double mutant treated with CI976, suggesting that the protein trafficking impairment was due to a critical PA concentration threshold. | |
| dc.description.sponsorship | Développment d'une infrastructure française distribuée coordonnée | en_US |
| dc.description.sponsorship | Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010 | en_US |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject.en | Acyltransferases | |
| dc.subject.en | Arabidopsis | |
| dc.subject.en | Arabidopsis Proteins | |
| dc.subject.en | Indoleacetic Acids | |
| dc.subject.en | Protein Transport | |
| dc.title.en | Lysophosphatidic acid acyltransferases: a link with intracellular protein trafficking in Arabidopsis root cells? | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1093/jxb/erab504 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale | en_US |
| dc.identifier.pubmed | 34982825 | en_US |
| bordeaux.journal | Journal of Experimental Botany | en_US |
| bordeaux.page | 1327-1343 | en_US |
| bordeaux.volume | 73 | en_US |
| bordeaux.hal.laboratories | Laboratoire de Biogenèse Membranaire (LBM) - UMR 5200 | en_US |
| bordeaux.issue | 5 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.identifier.funderID | Centre National de la Recherche Scientifique | en_US |
| bordeaux.identifier.funderID | Université de Bordeaux | en_US |
| bordeaux.identifier.funderID | Agence Nationale de la Recherche | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | hal-03795790 | |
| hal.version | 1 | |
| hal.date.transferred | 2022-10-04T09:01:59Z | |
| hal.export | true | |
| workflow.import.source | pubmed | |
| dc.rights.cc | Pas de Licence CC | en_US |
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