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dc.rights.licenseopenen_US
dc.contributor.authorDEJEAN, G.
hal.structure.identifierImmunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]
dc.contributor.authorTUDELA, Heloise
dc.contributor.authorBRUNO, L.
dc.contributor.authorKISSI, D.
dc.contributor.authorRAWADI, G.
dc.contributor.authorCLAUS, S.P.
dc.date.accessioned2022-05-03T11:24:24Z
dc.date.available2022-05-03T11:24:24Z
dc.date.issued2021-06-09
dc.identifier.issn2076-2607en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/139968
dc.description.abstractEnChristensenella minuta are human gut dwelling bacteria that have been proposed as key members of the gut microbiome, regulating energy balance and adiposity of their host. We formerly identified that a novel strain of C. minuta (strain DSM33407) boosted microbiota diversity and stimulated deconjugation of the primary bile acid taurocholic acid in human samples. However, there is no description of a bile salt hydrolase (BSH) protein carried in the genome of C. minuta. Here, we identified and cloned a protein from C. minuta’s genome that carries a potent BSH activity, which preferentially deconjugates glycine-conjugated bile acids. We then retrieved 14,319 putative BSH sequences from the NCBI database and filtered them using the UHGP database to collect a total of 6701 sequences that were used to build the most comprehensive phylogenetic tree of BSH-related enzymes identified in the human microbiome so far. This phylogenetic tree revealed that C. minuta’s BSH amino acid sequence clusters away from others with a threshold of 70% identity. This is therefore the first description of C. minuta’s BSH protein, which may be involved in its unique role within the human gut microbial ecosystem.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enBile acids
dc.subject.enBile salt hydrolase
dc.subject.enGut microbiome
dc.title.enIdentifying a novel bile salt hydrolase from the keystone gut bacterium christensenella minuta
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/microorganisms9061252en_US
dc.subject.halSciences du Vivant [q-bio]/Immunologieen_US
dc.identifier.pubmed34207623en_US
bordeaux.journalMicroorganismsen_US
bordeaux.volume9en_US
bordeaux.hal.laboratoriesImmunoConcEpT - UMR 5164en_US
bordeaux.issue6en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-03657835
hal.version1
hal.date.transferred2022-05-03T11:24:27Z
hal.exporttrue
dc.rights.ccCC BYen_US
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