Elemental maps in human allantochorial placental vessels cells: 1. High K$^{+}$ and acetylcholine effects
| hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
| dc.contributor.author | MICHELET-HABCHI, C. | |
| hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
| dc.contributor.author | BARBERET, P. | |
| hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
| dc.contributor.author | DUTTA, R.K. | |
| hal.structure.identifier | Physiologie et physiopathologie [PP] | |
| dc.contributor.author | GUIET-BARA, A. | |
| hal.structure.identifier | Physiologie et physiopathologie [PP] | |
| dc.contributor.author | BARA, M. | |
| hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
| dc.contributor.author | MORETTO, P. | |
| dc.contributor.editor | M. Takai and T. Kamiya | |
| dc.date.issued | 2003 | |
| dc.date.conference | 2002-09-08 | |
| dc.identifier.issn | 0168-583X | |
| dc.description.abstractEn | Regulation of vascular tone in the fetal extracorporeal circulation most likely depends on circulating hormones, local paracrine mechanisms and changes in membrane potential of vascular smooth muscle cells (VSMCs) and of vascular endothelial cells (VECs). The membrane potential is a function of the physiological activities of ionic channels (particularly, K$^{+}$ and Ca$^{2+}$ channels in these cells). These channels regulate the ionic distribution into these cells. Micro-particle induced X-ray emission (PIXE) analysis was applied to determine the ionic composition of VSMC and of VEC in the placental human allantochorial vessels in a physiological survival medium (Hanks' solution) modified by the addition of acetylcholine (ACh: which opens the calcium-sensitive K+ channels, KCa) and of high concentration of K+ (which blocks the voltage-sensitive K+ channels, Kdf). In VSMC (media layer), the addition of ACh induced no modification of the Na, K, Cl, P, S, Mg and Ca concentrations and high K+ medium increased significantly the Cl and K concentrations, the other ion concentrations remaining constant. In endothelium (VEC), ACh addition implicated a significant increase of Na and K concentration, and high K$^{+}$ medium, a significant increase in Cl and K concentration. These results indicated the importance of K$_{df}$, K$_{Ca}$ and KATP channels in the regulation of K+ intracellular distribution in VSMC and VEC and the possible intervention of a Na–K–2Cl cotransport and corroborated the previous electrophysiological data. | |
| dc.language.iso | en | |
| dc.publisher | Elsevier | |
| dc.source.title | Nuclear Instruments and Methods in Physics Research B | |
| dc.subject.en | Placental vessels | |
| dc.subject.en | Acetylcholine | |
| dc.subject.en | Ionic channels | |
| dc.subject.en | Microanalysis | |
| dc.subject.en | Micro-PIXE | |
| dc.subject.en | Scanning proton microprobe | |
| dc.title.en | Elemental maps in human allantochorial placental vessels cells: 1. High K$^{+}$ and acetylcholine effects | |
| dc.type | Communication dans un congrès | |
| dc.identifier.doi | 10.1016/S0168-583X(03)01045-0 | |
| dc.subject.hal | Physique [physics]/Physique [physics]/Physique Médicale [physics.med-ph] | |
| bordeaux.journal | Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms | |
| bordeaux.page | 354-358 | |
| bordeaux.volume | 210 | |
| bordeaux.country | JP | |
| bordeaux.title.proceeding | Nuclear Instruments and Methods in Physics Research B | |
| bordeaux.conference.city | Takasaki | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | in2p3-00103409 | |
| hal.version | 1 | |
| hal.invited | non | |
| hal.proceedings | oui | |
| hal.conference.end | 2002-09-13 | |
| hal.popular | non | |
| hal.audience | Non spécifiée | |
| hal.origin.link | https://hal.archives-ouvertes.fr//in2p3-00103409v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.btitle=Nuclear%20Instruments%20and%20Methods%20in%20Physics%20Research%20B&rft.jtitle=Nuclear%20Instruments%20and%20Methods%20in%20Physics%20Research%20Section%20B:%20Beam%20Interactions%20with%20Materials%20and%20Atoms&rft.date=2003&rft.volume=210&rft.spage=354-358&rft.epage=354-358&rft.eissn=0168-583X&rft.issn=0168-583X&rft.au=MICHELET-HABCHI,%20C.&BARBERET,%20P.&DUTTA,%20R.K.&GUIET-BARA,%20A.&BARA,%20M.&rft.genre=unknown |
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