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hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorHABCHI, C.
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorNGUYEN, D.T.
hal.structure.identifierChimie Nucléaire Analytique et Bio-environnementale [CNAB]
dc.contributor.authorDEVÈS, Guillaume
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorINCERTI, S.
hal.structure.identifierLaboratoire de Sciences de la Terre [LST]
dc.contributor.authorLEMELLE, L.
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorLE VAN VANG, P.
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorMORETTO, Ph.
hal.structure.identifierChimie Nucléaire Analytique et Bio-environnementale [CNAB]
dc.contributor.authorORTEGA, R.
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorSEZNEC, H.
dc.contributor.authorSAKELLARIOU, A.
hal.structure.identifierChimie Nucléaire Analytique et Bio-environnementale [CNAB]
dc.contributor.authorSERGEANT, C.
hal.structure.identifierLaboratoire de Sciences de la Terre [LST]
dc.contributor.authorSIMIONOVICI, A.
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorYNSA, M.D.
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorGONTIER, E.
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorHEISS, M.
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorPOUTHIER, T.
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBOUDOU, A.
hal.structure.identifierLaboratoire des Composites Thermostructuraux [LCTS]
dc.contributor.authorREBILLAT, F.
dc.date.issued2006
dc.date.conference2005-06-27
dc.description.abstractEnScanning transmission ion microscopy tomography (STIM-T) was carried out on diatom cells with the aim of displaying their 3D structure and performing density measurements on their silica skeleton. Two software packages were compared for data reduction: TomoRebuild, based on a simple filtered backprojection algorithm, and DISRA, an iterative program. Silicon carbide microfibres of known density were also analysed as reference specimens. Similar results were obtained with both algorithms, demonstrating the ability of STIM-T to provide density measurements at the cell level without requiring any standard calibration samples. This unique feature stresses the interest of STIM-T to accurately normalise X ray emission micro-tomography data from synchrotron radiation (SXRF: synchrotron radiation X-ray fluorescence) or ion beam sources (PIXE: particle induced X-ray emission). Possible enhancements of the DISRA code are discussed in order to facilitate its use for the reconstruction of future PIXE/STIM tomography data. A “nanoprobe” coupled to a Singletron® accelerator, allowing a spatial resolution of a few tens of nanometers, is going to be built in the coming months at the Centre d'Etudes Nucléaires de Bordeaux Gradignan (CENBG). This new facility will bring promising applications in imaging and analysis at the sub-cellular level.
dc.language.isoen
dc.subject.enScanning transmission ion microscopy
dc.subject.enComputed tomography
dc.subject.enMicro-tomography
dc.subject.enDiatom cell
dc.subject.en3D imaging
dc.subject.enNon-destructive characterisation
dc.subject.enNuclear microprobe
dc.title.enThree-dimensional densitometry imaging of diatom cells using STIM tomography
dc.typeCommunication dans un congrès
dc.identifier.doi10.1016/j.nimb.2006.03.074
dc.subject.halPhysique [physics]/Physique [physics]/Biophysique [physics.bio-ph]
bordeaux.page653-659
bordeaux.volume249
bordeaux.countryES
bordeaux.conference.citySeville
bordeaux.peerReviewedoui
hal.identifierin2p3-00426830
hal.version1
hal.invitedoui
hal.proceedingsoui
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//in2p3-00426830v1
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