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Combined use of hard X-ray phase contrast imaging and X-ray fluorescence microscopy for sub-cellular metal quantification.

hal.structure.identifierEuropean Synchrotron Radiation Facility [ESRF]
dc.contributor.authorKOSIOR, Ewelina
hal.structure.identifierINSERM U836, équipe 6, Rayonnement synchrotron et recherche médicale
dc.contributor.authorBOHIC, Sylvain
hal.structure.identifierEuropean Synchrotron Radiation Facility [ESRF]
dc.contributor.authorSUHONEN, Heikki
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorORTEGA, Richard
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorDEVÈS, Guillaume
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorCARMONA, Asuncion
hal.structure.identifierCP
dc.contributor.authorMARCHI, Florence
hal.structure.identifierSurface Science Laboratory
dc.contributor.authorGUILLET, Jean Francois
hal.structure.identifierEuropean Synchrotron Radiation Facility [ESRF]
dc.contributor.authorCLOETENS, Peter
dc.date.issued2012-02
dc.identifier.issn1047-8477
dc.description.abstractEnHard X-ray fluorescence microscopy and magnified phase contrast imaging are combined to obtain quantitative maps of the projected metal concentration in whole cells. The experiments were performed on freeze dried cells at the nano-imaging station ID22NI of the European Synchrotron Radiation Facility (ESRF). X-ray fluorescence analysis gives the areal mass of most major, minor and trace elements; it is validated using a biological standard of known composition. Quantitative phase contrast imaging provides maps of the projected mass and is validated using calibration samples and through comparison with Atomic Force Microscopy and Scanning Transmission Ion Microscopy. Up to now, absolute quantification at the sub-cellular level was impossible using X-ray fluorescence microscopy but can be reached with the use of the proposed approach.
dc.language.isoen
dc.publisherElsevier
dc.subject.enX-ray fluorescence
dc.subject.enphase contrast imaging
dc.subject.ensynchrotron
dc.subject.entrace elements
dc.subject.meshAlgorithms
dc.subject.meshAnimals
dc.subject.meshReference Standards
dc.subject.meshSingle-Cell Analysis
dc.subject.meshTrace Elements
dc.subject.meshX-Rays
dc.subject.meshZinc
dc.subject.meshCalibration
dc.subject.meshCell Nucleus
dc.subject.meshMicroscopy, Fluorescence
dc.subject.meshMicroscopy, Phase-Contrast
dc.subject.meshPC12 Cells
dc.subject.meshParticle Size
dc.subject.meshPotassium
dc.subject.meshRats
dc.title.enCombined use of hard X-ray phase contrast imaging and X-ray fluorescence microscopy for sub-cellular metal quantification.
dc.typeArticle de revue
dc.identifier.doi10.1016/j.jsb.2011.12.005
dc.subject.halSciences du Vivant [q-bio]/Ingénierie biomédicale/Médecine nucléaire
bordeaux.journalJournal of Structural Biology
bordeaux.page239-47
bordeaux.volume177
bordeaux.issue2
bordeaux.peerReviewedoui
hal.identifierinserm-00662829
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-00662829v1
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