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Different in vitro exposure regimens of murine primary macrophages to silver nanoparticles induce different fates of nanoparticles and different toxicological and functional consequences.

hal.structure.identifierLaboratoire de Chimie et Biologie des Métaux [LCBM - UMR 5249]
dc.contributor.authorAUDE-GARCIA, Catherine
hal.structure.identifierPhysiologie cellulaire et végétale [LPCV]
dc.contributor.authorVILLIERS, Florent
hal.structure.identifierLaboratoire de Chimie et Biologie des Métaux [LCBM - UMR 5249]
dc.contributor.authorCOLLIN-FAURE, Véronique
hal.structure.identifier[GIN] Grenoble Institut des Neurosciences [GIN]
dc.contributor.authorPERNET-GALLAY, Karin
hal.structure.identifierLaboratoire d'Etude des Matériaux par Microscopie Avancée [LEMMA ]
dc.contributor.authorJOUNEAU, Pierre-Henri
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorSORIEUL, Stéphanie
hal.structure.identifierLaboratoire de Chimie et Biologie des Métaux [LCBM - UMR 5249]
dc.contributor.authorMURE, Geoffrey
hal.structure.identifierLaboratoire Edifices Nanométriques [LEDNA]
dc.contributor.authorGERDIL, Adèle
hal.structure.identifierLaboratoire Edifices Nanométriques [LEDNA]
dc.contributor.authorHERLIN-BOIME, Nathalie
hal.structure.identifierLaboratoire Lésions des Acides Nucléiques [LAN]
dc.contributor.authorCARRIÈRE, Marie
hal.structure.identifierLaboratoire de Chimie et Biologie des Métaux [LCBM - UMR 5249]
dc.contributor.authorRABILLOUD, Thierry
dc.date.created2015
dc.date.issued2016
dc.identifier.issn1743-5390
dc.description.abstractEnSilver nanoparticles (Ag-NPs) are used in a variety of consumers' goods. Their toxicological impact is currently intensely studied, mostly upon acute exposure, but their intracellular dissolution and fate is rather poorly documented. In this study, murine primary macrophages were exposed to a single high but non-lethal dose of Ag-NPs or to repeated, low doses of Ag-NPs. Cells were either collected immediately after acute exposure or after 72 h of recovery in the NP-free exposure medium. Ag intracellular content and distribution were analyzed by particle-induced X-ray emission, transmission electron microscopy coupled to energy-dispersive spectroscopy analysis and inductively coupled plasma mass spectrometry. In parallel, macrophage functionality as well as inflammatory and thiol-responses were assessed after Ag-NP exposure. We show that Ag accumulation in macrophages is similar upon acute and repeated exposure to Ag-NPs, and that Ag is partly expelled from cells during the 72 h recovery stage. However, acute exposure leads to a strong response of macrophages, characterized by reduced mitochondrial membrane potential, phagocytic capacity and nitric oxide (NO) production upon lipopolysaccharide (LPS) stimulation. Under this condition, we also show an increased release of proinflammatory cytokines as well as a decreased release of anti-inflammatory cytokines. This response is reversible since these biomarkers reach their basal level after the recovery phase; and is much less intense in repeatedly exposed cells. These results suggest that repeated exposure of macrophages to Ag-NPs, which is a more realistic exposure scenario than acute exposure, leads to significant Ag intracellular accumulation but a much less intense toxicological response
dc.description.sponsorshipINITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE
dc.description.sponsorshipPlateforme d'identification des nanoparticules dédiée à la sécurité
dc.language.isoen
dc.publisherTaylor & Francis
dc.subject.enToxicity
dc.subject.enSilver
dc.subject.enNanoparticle
dc.subject.enMurine
dc.subject.enMacrophage
dc.subject.enMice
dc.subject.enSpeciation
dc.title.enDifferent in vitro exposure regimens of murine primary macrophages to silver nanoparticles induce different fates of nanoparticles and different toxicological and functional consequences.
dc.typeArticle de revue
dc.identifier.doi10.3109/17435390.2015.1104738
dc.subject.halSciences du Vivant [q-bio]/Toxicologie
bordeaux.journalNanotoxicology
bordeaux.page586-596
bordeaux.volume10
bordeaux.issue5
bordeaux.peerReviewedoui
hal.identifierhal-01271894
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01271894v1
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