Afficher la notice abrégée

Assessment of Radio-Induced Damage in Endothelial Cells Irradiated with 40 kVp, 220 kVp, and 4 MV X-rays by Means of Micro and Nanodosimetric Calculations

hal.structure.identifierInstitut de Radioprotection et de Sûreté Nucléaire [IRSN]
dc.contributor.authorTANG, Nicolas
hal.structure.identifierInstitut de Radioprotection et de Sûreté Nucléaire [IRSN]
dc.contributor.authorBUENO, Marta
dc.contributor.authorMEYLAN, Sylvain
hal.structure.identifierInstitut de Radioprotection et de Sûreté Nucléaire [IRSN]
dc.contributor.authorPERROT, Yann
hal.structure.identifierInstitut de Radioprotection et de Sûreté Nucléaire [IRSN]
dc.contributor.authorTRAN, Hoang N.
hal.structure.identifierInstitut de Radioprotection et de Sûreté Nucléaire [IRSN]
dc.contributor.authorFRENEAU, Amélie
hal.structure.identifierInstitut de Radioprotection et de Sûreté Nucléaire [IRSN]
dc.contributor.authorDOS SANTOS, Morgane
hal.structure.identifierInstitut de Radioprotection et de Sûreté Nucléaire [IRSN]
dc.contributor.authorVAURIJOUX, Aurélie
hal.structure.identifierInstitut de Radioprotection et de Sûreté Nucléaire [IRSN]
dc.contributor.authorGRUEL, Gaëtan
dc.contributor.authorBERNAL, Mario A.
hal.structure.identifierCentre de Recherches en Cancérologie de Toulouse [CRCT]
dc.contributor.authorBORDAGE, Marie-Claude
dc.contributor.authorEMFIETZOGLOU, Dimitris
dc.contributor.authorFRANCIS, Ziad
dc.contributor.authorGUATELLI, Susanna
dc.contributor.authorIVANCHENKO, Vladimir
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorKARAMITROS, Mathieu
dc.contributor.authorKYRIAKOU, Ioanna
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorSHIN, Wook-Geun
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorINCERTI, Sébastien
hal.structure.identifierInstitut de Radioprotection et de Sûreté Nucléaire [IRSN]
dc.contributor.authorVILLAGRASA, Carmen
dc.date.issued2019
dc.description.abstractEn<jats:p>The objective of this work was to study the differences in terms of early biological effects that might exist between different X-rays energies by using a mechanistic approach. To this end, radiobiological experiments exposing cell monolayers to three X-ray energies were performed in order to assess the yields of early DNA damage, in particular of double-strand breaks (DSBs). The simulation of these irradiations was set in order to understand the differences in the obtained experimental results. Hence, simulated results in terms of microdosimetric spectra and early DSB induction were analyzed and compared to the experimental data. Human umbilical vein endothelial cells (HUVECs) were irradiated with 40, 220 kVp, and 4 MV X-rays. The Geant4 Monte Carlo simulation toolkit and its extension Geant4-DNA were used for the simulations. Microdosimetric calculations aiming to determine possible differences in the variability of the energy absorbed by the irradiated cell population for those photon spectra were performed on 10,000 endothelial cell nuclei representing a cell monolayer. Nanodosimetric simulations were also carried out using a computation chain that allowed the simulation of physical, physico-chemical, and chemical stages on a single realistic endothelial cell nucleus model including both heterochromatin and euchromatin. DNA damage was scored in terms of yields of prompt DSBs per Gray (Gy) and per giga (109) base pair (Gbp) and DSB complexity was derived in order to be compared to experimental data expressed as numbers of histone variant H2AX (γ-H2AX) foci per cell. The calculated microdosimetric spread in the irradiated cell population was similar when comparing between 40 and 220 kVp X-rays and higher when comparing with 4 MV X-rays. Simulated yields of induced DSB/Gy/Gbp were found to be equivalent to those for 40 and 220 kVp but larger than those for 4 MV, resulting in a relative biological effectiveness (RBE) of 1.3. Additionally, DSB complexity was similar between the considered photon spectra. Simulated results were in good agreement with experimental data obtained by IRSN (Institut de radioprotection et de sûreté nucléaire) radiobiologists. Despite differences in photon energy, few differences were observed when comparing between 40 and 220 kVp X-rays in microdosimetric and nanodosimetric calculations. Nevertheless, variations were observed when comparing between 40/220 kVp and 4 MV X-rays. Thanks to the simulation results, these variations were able to be explained by the differences in the production of secondary electrons with energies below 10 keV.</jats:p>
dc.language.isoen
dc.title.enAssessment of Radio-Induced Damage in Endothelial Cells Irradiated with 40 kVp, 220 kVp, and 4 MV X-rays by Means of Micro and Nanodosimetric Calculations
dc.typeArticle de revue
dc.identifier.doi10.3390/ijms20246204
dc.subject.halPhysique [physics]
bordeaux.journalInt.J.Mol.Sci.
bordeaux.page6204
bordeaux.volume20
bordeaux.issue24
bordeaux.peerReviewedoui
hal.identifierhal-02416923
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02416923v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Int.J.Mol.Sci.&amp;rft.date=2019&amp;rft.volume=20&amp;rft.issue=24&amp;rft.spage=6204&amp;rft.epage=6204&amp;rft.au=TANG,%20Nicolas&amp;BUENO,%20Marta&amp;MEYLAN,%20Sylvain&amp;PERROT,%20Yann&amp;TRAN,%20Hoang%20N.&amp;rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée