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A correlative light electron microscopy approach reveals plasmodesmata ultrastructure at the graft interface

dc.rights.licenseopenen_US
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorCHAMBAUD, Clement
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorCOOKSON, Sarah
IDREF: 22161009X
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorOLLAT, Nathalie
IDREF: 126740062
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorBAYER, Emmanuelle
dc.contributor.authorBROCARD, Lysiane
dc.date.accessioned2022-03-09T17:46:46Z
dc.date.available2022-03-09T17:46:46Z
dc.date.issued2022-01-01
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/136414
dc.description.abstractEnAbstract Despite recent progress in our understanding of graft union formation, we still know little about the cellular events underlying the grafting process. This is partially due to the difficulty of reliably targeting the graft interface in electron microscopy to study its ultrastructure and three-dimensional architecture. To overcome this technological bottleneck, we developed a correlative light electron microscopy (CLEM) approach to study the graft interface with high ultrastructural resolution. Grafting hypocotyls of Arabidopsis thaliana lines expressing yellow FP or monomeric red FP in the endoplasmic reticulum (ER) allowed efficient targeting of the grafting interface for examination under light and electron microscopy. To explore the potential of our method to study sub-cellular events at the graft interface, we focused on the formation of secondary plasmodesmata (PD) between the grafted partners. We showed that four classes of PD were formed at the interface and that PD introgression into the cell wall was initiated equally by both partners. Moreover, the success of PD formation appeared not systematic with a third of PD not spanning the cell wall entirely. Characterizing the ultrastructural characteristics of these incomplete PD gives us insights into the process of secondary PD biogenesis. We found that the establishment of successful symplastic connections between the scion and rootstock occurred predominantly in the presence of thin cell walls and ER–plasma membrane tethering. The resolution reached in this work shows that our CLEM method advances the study of biological processes requiring the combination of light and electron microscopy.
dc.language.isoENen_US
dc.subject.enCell biology
dc.title.enA correlative light electron microscopy approach reveals plasmodesmata ultrastructure at the graft interface
dc.typeArticle de revueen_US
dc.identifier.doi10.1093/plphys/kiab485en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
dc.identifier.pubmed34687300en_US
bordeaux.journalPlant Physiologyen_US
bordeaux.page44-55
bordeaux.volume188
bordeaux.hal.laboratoriesLaboratoire de Biogenèse Membranaire (LBM) - UMR 5200en_US
bordeaux.issue1
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.exportfalse
dc.rights.ccPas de Licence CCen_US
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