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Proteomic Profiling of Hepatocellular Adenomas Paves the Way to Diagnostic and Prognostic Approaches

dc.rights.licenseopenen_US
hal.structure.identifierBordeaux Research In Translational Oncology [Bordeaux] [BaRITOn]
dc.contributor.authorDOURTHE, Cyril
hal.structure.identifierBordeaux Research In Translational Oncology [Bordeaux] [BaRITOn]
dc.contributor.authorJULIEN, Celine
hal.structure.identifierBordeaux Research In Translational Oncology [Bordeaux] [BaRITOn]
dc.contributor.authorDI-TOMMASO, Sylvaine
hal.structure.identifierPlateforme Protéome [Bordeaux]
dc.contributor.authorDUPUY, Jean-William
dc.contributor.authorDUGOT-SENANT, Nathalie
hal.structure.identifierNeurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]
dc.contributor.authorBROCHARD, Alexandre
hal.structure.identifierBordeaux Research In Translational Oncology [Bordeaux] [BaRITOn]
dc.contributor.authorLE BAIL, Brigitte
hal.structure.identifierBordeaux Research In Translational Oncology [Bordeaux] [BaRITOn]
dc.contributor.authorBLANC, Jean Frederic
hal.structure.identifierBordeaux Research In Translational Oncology [Bordeaux] [BaRITOn]
dc.contributor.authorCHICHE, Laurence
hal.structure.identifierBordeaux Research In Translational Oncology [Bordeaux] [BaRITOn]
dc.contributor.authorBALABAUD, Charles
hal.structure.identifierBordeaux Research In Translational Oncology [Bordeaux] [BaRITOn]
dc.contributor.authorBIOULAC-SAGE, Paulette
hal.structure.identifierBordeaux Research In Translational Oncology [Bordeaux] [BaRITOn]
dc.contributor.authorSALTEL, Frédéric
ORCID: 0000-0002-0724-9680
IDREF: 089053079
hal.structure.identifierBordeaux Research In Translational Oncology [Bordeaux] [BaRITOn]
dc.contributor.authorRAYMOND, Anne-Aurelie
dc.date.accessioned2021-12-10T09:19:02Z
dc.date.available2021-12-10T09:19:02Z
dc.date.issued2021-09
dc.identifier.issn1527-3350en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/124105
dc.description.abstractEnBackground and Aims : Through an exploratory proteomic approach based on typical hepatocellular adenomas (HCAs), we previously identified a diagnostic biomarker for a distinctive subtype of HCA with high risk of bleeding, already validated on a multicenter cohort. We hypothesized that the whole protein expression deregulation profile could deliver much more informative data for tumor characterization. Therefore, we pursued our analysis with the characterization of HCA proteomic profiles, evaluating their correspondence with the established genotype/phenotype classification and assessing whether they could provide added diagnosis and prognosis values. Approach and Results : From a collection of 260 cases, we selected 52 typical cases of all different subgroups on which we built a reference HCA proteomics database. Combining laser microdissection and mass-spectrometry–based proteomic analysis, we compared the relative protein abundances between tumoral (T) and nontumoral (NT) liver tissues from each patient and we defined a specific proteomic profile of each of the HCA subgroups. Next, we built a matching algorithm comparing the proteomic profile extracted from a patient with our reference HCA database. Proteomic profiles allowed HCA classification and made diagnosis possible, even for complex cases with immunohistological or genomic analysis that did not lead to a formal conclusion. Despite a well-established pathomolecular classification, clinical practices have not substantially changed and the HCA management link to the assessment of the malignant transformation risk remains delicate for many surgeons. That is why we also identified and validated a proteomic profile that would directly evaluate malignant transformation risk regardless of HCA subtype. Conclusions : This work proposes a proteomic-based machine learning tool, operational on fixed biopsies, that can improve diagnosis and prognosis and therefore patient management for HCAs.
dc.language.isoENen_US
dc.title.enProteomic Profiling of Hepatocellular Adenomas Paves the Way to Diagnostic and Prognostic Approaches
dc.typeArticle de revueen_US
dc.identifier.doi10.1002/hep.31826en_US
dc.subject.halSciences du Vivant [q-bio]/Neurosciences [q-bio.NC]en_US
dc.identifier.pubmed33754354en_US
dc.description.sponsorshipEuropeEuropean Funds for Regional Development (Feder), N° Presage 32078en_US
bordeaux.journalHepatologyen_US
bordeaux.page1595-1610en_US
bordeaux.volume74en_US
bordeaux.hal.laboratoriesNeurocentre Magendie - UMR-S 1215en_US
bordeaux.issue3en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-03474232
hal.version1
hal.date.transferred2021-12-10T09:19:05Z
hal.exporttrue
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Hepatology&rft.date=2021-09&rft.volume=74&rft.issue=3&rft.spage=1595-1610&rft.epage=1595-1610&rft.eissn=1527-3350&rft.issn=1527-3350&rft.au=DOURTHE,%20Cyril&JULIEN,%20Celine&DI-TOMMASO,%20Sylvaine&DUPUY,%20Jean-William&DUGOT-SENANT,%20Nathalie&rft.genre=article


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