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Knockout of VvCCD8 gene in grapevine affects shoot branching.

dc.rights.licenseopenen_US
dc.contributor.authorREN, Chong
dc.contributor.authorGUO, Yuchen
dc.contributor.authorKONG, Junhua
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorLECOURIEUX, Fatma
IDREF: 158692306
dc.contributor.authorDAI, Zhanwu
IDREF: 22822473X
dc.contributor.authorLI, Shaohua
dc.contributor.authorLIANG, Zhenchang
dc.date.accessioned2020-09-11T12:21:34Z
dc.date.available2020-09-11T12:21:34Z
dc.date.issued2020-01-29
dc.identifier.issn1471-2229en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/11300
dc.description.abstractEnShoot branching is an important trait of plants that allows them to adapt to environment changes. Strigolactones (SLs) are newly identified plant hormones that inhibit shoot branching in plants. The SL biosynthesis genes CCD7 (carotenoid cleavage dioxygenase 7) and CCD8 have been found to regulate branching in several herbaceous plants by taking advantage of their loss-of-function mutants. However, the role for CCD7 and CCD8 in shoot branching control in grapevine is still unknown due to the lack of corresponding mutants. Here we employed the CRISPR/Cas9 system to edit the VvCCD7 and VvCCD8 genes in the grape hybrid 41B. The 41B embryogenic cells can easily be transformed and used for regeneration of the corresponding transformed plants. Sequencing analysis revealed that gene editing has been used successfully to target both VvCCD genes in 41B embryogenic cells. After regeneration, six 41B plantlets were identified as transgenic plants carrying the CCD8-sgRNA expression cassette. Among these, four plants showed mutation in the target region and were selected as ccd8 mutants. These ccd8 mutants showed increased shoot branching compared to the corresponding wild-type plants. In addition, no off-target mutation was detected in the tested mutants at predicted off-target sites. Our results underline the key role of VvCCD8 in the control of grapevine shoot branching.
dc.language.isoENen_US
dc.subjectArabidopsis Proteins
dc.subjectCRISPR-Cas Systems
dc.subjectDioxygenases
dc.subjectGene Editing
dc.subjectGene Knockout Techniques
dc.subjectGenes
dc.subjectPlant
dc.subjectPlant Shoots
dc.subjectPlants
dc.subjectGenetically Modified
dc.subjectVitis
dc.subject.enCCD8
dc.subject.enCRISPR/Cas9
dc.subject.enMutant
dc.subject.enShoot branching
dc.subject.enStrigolactone
dc.title.enKnockout of VvCCD8 gene in grapevine affects shoot branching.
dc.title.alternativeBMC Plant Biolen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1186/s12870-020-2263-3en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
dc.identifier.pubmed31996144en_US
bordeaux.journalBMC Plant Biologyen_US
bordeaux.page47en_US
bordeaux.volume20en_US
bordeaux.hal.laboratoriesEcophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287en_US
bordeaux.issue1en_US
bordeaux.institutionBordeaux Sciences Agroen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.exportfalse
workflow.import.sourcepubmed
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=BMC%20Plant%20Biology&rft.date=2020-01-29&rft.volume=20&rft.issue=1&rft.spage=47&rft.epage=47&rft.eissn=1471-2229&rft.issn=1471-2229&rft.au=REN,%20Chong&GUO,%20Yuchen&KONG,%20Junhua&LECOURIEUX,%20Fatma&DAI,%20Zhanwu&rft.genre=article


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