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In vivo online magnetic resonance quantification of absolute metabolite concentrations in microdialysate

hal.structure.identifierInstitut des Sciences Moléculaires [ISM]
dc.contributor.authorGLÖGGLER, Stefan
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorRIZZITELLI, Silvia
hal.structure.identifierInstitut des Sciences Moléculaires [ISM]
dc.contributor.authorPINAUD, Noël
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorRAFFARD, Gérard
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorZHENDRE, Vanessa
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorBOUCHAUD, Véronique
hal.structure.identifierInstitut de recherche en informatique de Toulouse [IRIT]
dc.contributor.authorSANCHEZ, Stephane
hal.structure.identifierService NEUROSPIN [NEUROSPIN]
dc.contributor.authorRADECKI, Guillaume
hal.structure.identifierService NEUROSPIN [NEUROSPIN]
dc.contributor.authorCIOBANU, Luisa
hal.structure.identifierLaboratoire Structure et Dynamique par Résonance Magnétique (LCF) [LSDRM]
dc.contributor.authorWONG, Alan
hal.structure.identifierCentre de Recherche en Acquisition et Traitement de l'Image pour la Santé [CREATIS]
dc.contributor.authorCREMILLIEUX, Yannick
dc.date.accessioned2021-10-07T16:29:37Z
dc.date.available2021-10-07T16:29:37Z
dc.date.issued2016-12
dc.identifier.issn2045-2322
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/112659
dc.description.abstractEnIn order to study metabolic processes in animal models of diseases and in patients, microdialysis probes have evolved as powerful tools that are minimally invasive. However, analyses of microdialysate, performed remotely, do not provide real-time monitoring of microdialysate composition. Microdialysate solutions can theoretically be analyzed online inside a preclicinal or clinical MRI scanner using MRS techniques. Due to low NMR sensitivity, acquisitions of real-time NMR spectra on very small solution volumes (μL) with low metabolite concentrations (mM range) represent a major issue. To address this challenge we introduce the approach of combining a microdialysis probe with a custom-built magnetic resonance microprobe that allows for online metabolic analysis (1 H and 13 C) with high sensitivity under continuous flow conditions. This system is mounted inside an MRI scanner and allows performing simultaneously MRI experiments and rapid MRS metabolic analysis of the microdialysate. The feasibility of this approach is demonstrated by analyzing extracellular brain cancer cells (glioma) in vitro and brain metabolites in an animal model in vivo. We expect that our approach is readily translatable into clinical settings and can be used for a better and precise understanding of diseases linked to metabolic dysfunction.
dc.language.isoen
dc.publisherNature Publishing Group
dc.title.enIn vivo online magnetic resonance quantification of absolute metabolite concentrations in microdialysate
dc.typeArticle de revue
dc.identifier.doi10.1038/srep36080
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalScientific Reports
bordeaux.volume6
bordeaux.hal.laboratoriesCentre de Résonance Magnétique des Systèmes Biologiques (CRMSB) - UMR 5536*
bordeaux.issue1
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-02375690
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02375690v1
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