An elastase activity reporter for Electronic Paramagnetic Resonance (EPR) and Overhauser-enhanced Magnetic Resonance Imaging (OMRI) as a line-shifting nitroxide
hal.structure.identifier | Centre de résonance magnétique des systèmes biologiques [CRMSB] | |
dc.contributor.author | JUGNIOT, Natacha | |
hal.structure.identifier | Institut de Chimie Radicalaire [ICR] | |
dc.contributor.author | DUTTAGUPTA, Indranil | |
hal.structure.identifier | Centre de résonance magnétique des systèmes biologiques [CRMSB] | |
dc.contributor.author | RIVOT, Angelique | |
hal.structure.identifier | Centre de résonance magnétique des systèmes biologiques [CRMSB] | |
dc.contributor.author | MASSOT, Philippe | |
hal.structure.identifier | Centre de résonance magnétique des systèmes biologiques [CRMSB] | |
dc.contributor.author | CARDIET, Colleen | |
hal.structure.identifier | Institut de Génomique Fonctionnelle [IGF] | |
dc.contributor.author | PIZZOCCARO, Anne | |
hal.structure.identifier | Institut des Sciences Moléculaires de Marseille [ISM2] | |
dc.contributor.author | JEAN, Marion | |
hal.structure.identifier | Institut des Sciences Moléculaires de Marseille [ISM2] | |
dc.contributor.author | VANTHUYNE, Nicolas | |
hal.structure.identifier | Centre de résonance magnétique des systèmes biologiques [CRMSB] | |
dc.contributor.author | FRANCONI, Jean-Michel | |
hal.structure.identifier | Université Grenoble Alpes - UFR Arts & Sciences Humaines [UGA UFR ARSH] | |
dc.contributor.author | VOISIN, Pierre | |
dc.contributor.author | DEVOUASSOUX, Gilles | |
hal.structure.identifier | Centre de résonance magnétique des systèmes biologiques [CRMSB] | |
dc.contributor.author | PARZY, Elodie | |
hal.structure.identifier | Centre de résonance magnétique des systèmes biologiques [CRMSB] | |
dc.contributor.author | THIAUDIÈRE, Eric | |
hal.structure.identifier | Institut de Chimie Radicalaire [ICR] | |
dc.contributor.author | MARQUE, Sylvain R.A. | |
hal.structure.identifier | Physiopathologie de l'immunodépression associée aux réponses inflammatoires systémiques / Pathophysiology of Injury-induced Immunosuppression [PI3] | |
dc.contributor.author | BENTAHER, Abderrazzak | |
hal.structure.identifier | Institut de Chimie Radicalaire [ICR] | |
dc.contributor.author | AUDRAN, Gérard | |
hal.structure.identifier | Centre de résonance magnétique des systèmes biologiques [CRMSB] | |
dc.contributor.author | MELLET, Philippe | |
dc.date.accessioned | 2021-10-07T16:29:00Z | |
dc.date.available | 2021-10-07T16:29:00Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0891-5849 | |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/112626 | |
dc.description.abstractEn | Pulmonary inflammatory diseases are a major burden worldwide. They have in common an influx of neutrophils. Neutrophils secrete unchecked proteases at inflammation sites consequently leading to a protease/inhibitor imbalance. Among these proteases, neutrophil elastase is responsible for the degradation of the lung structure via elastin fragmentation. Therefore, monitoring the protease/inhibitor status in lungs non-invasively would be an important diagnostic tool.Herein we present the synthesis of a MeO-Suc-(Ala)2-Pro-Val-nitroxide, a line-shifting elastase activity probe suitable for Electron Paramagnetic Resonance spectroscopy (EPR) and Overhauser-enhanced Magnetic Resonance Imaging (OMRI). It is a fast and sensitive neutrophil elastase substrate with Km = 15 ± 2.9 µM, kcat/Km = 930,000 s−1 M−1 and Km = 25 ± 5.4 µM, kcat/Km = 640,000 s−1 M−1 for the R and S isomers, respectively. These properties are suitable to detect accurately concentrations of neutrophil elastase as low as 1 nM. The substrate was assessed with broncho-alveolar lavages samples derived from a mouse model of Pseudomonas pneumonia. Using EPR spectroscopy we observed a clear-cut difference between wild type animals and animals deficient in neutrophil elastase or deprived of neutrophil Elastase, Cathepsin G and Proteinase 3 or non-infected animals.These results provide new preclinical ex vivo and in vivo diagnostic methods. They can lead to clinical methods to promote in time lung protection. | |
dc.language.iso | en | |
dc.publisher | Elsevier | |
dc.subject.en | Nitroxide | |
dc.subject.en | EPR | |
dc.subject.en | Protease | |
dc.subject.en | OMRI | |
dc.subject.en | Peptide | |
dc.subject.en | Molecular imaging | |
dc.subject.en | Inflammation | |
dc.title.en | An elastase activity reporter for Electronic Paramagnetic Resonance (EPR) and Overhauser-enhanced Magnetic Resonance Imaging (OMRI) as a line-shifting nitroxide | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1016/j.freeradbiomed.2018.08.006 | |
dc.subject.hal | Sciences du Vivant [q-bio]/Ingénierie biomédicale/Imagerie | |
dc.subject.hal | Chimie/Chimie organique | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM] | |
bordeaux.journal | Free Radical Biology and Medicine | |
bordeaux.page | 101-112 | |
bordeaux.volume | 126 | |
bordeaux.hal.laboratories | Centre de Résonance Magnétique des Systèmes Biologiques (CRMSB) - UMR 5536 | * |
bordeaux.institution | Université de Bordeaux | |
bordeaux.institution | CNRS | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-02421179 | |
hal.version | 1 | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-02421179v1 | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Free%20Radical%20Biology%20and%20Medicine&rft.date=2018&rft.volume=126&rft.spage=101-112&rft.epage=101-112&rft.eissn=0891-5849&rft.issn=0891-5849&rft.au=JUGNIOT,%20Natacha&DUTTAGUPTA,%20Indranil&RIVOT,%20Angelique&MASSOT,%20Philippe&CARDIET,%20Colleen&rft.genre=article |
Files in this item
Files | Size | Format | View |
---|---|---|---|
There are no files associated with this item. |