Mostrar el registro sencillo del ítem
Nasal airway epithelial cell IL-6 and FKBP51 gene expression and steroid sensitivity in asthmatic children
| dc.rights.license | open | en_US |
| hal.structure.identifier | Centre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB] | |
| dc.contributor.author | FAYON, Michael | |
| hal.structure.identifier | Centre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB] | |
| dc.contributor.author | LACOSTE-RODRIGUES, Aurelie | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | BARAT, Pascal | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | HELBLING, Jean Christophe | |
| dc.contributor.author | NACKA, Fabienne | |
| hal.structure.identifier | Centre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB] | |
| dc.contributor.author | BERGER, Patrick
ORCID: 0000-0003-4702-0343 IDREF: 060717998 | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | MOISAN, Marie Pierre
IDREF: 060242264 | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | CORCUFF, Jean-Benoit
IDREF: 058708154 | |
| dc.date.accessioned | 2021-09-28T09:34:48Z | |
| dc.date.available | 2021-09-28T09:34:48Z | |
| dc.date.issued | 2017 | |
| dc.identifier.issn | 1932-6203 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/112458 | |
| dc.description.abstractEn | Background: Many asthmatic patients exhibit uncontrolled asthma despite high-dose inhaled corticosteroids (ICS). Airway epithelial cells (AEC) have distinct activation profiles that can influence ICS response. Objectives: A pilot study to identify gene expression markers of AEC dysfunction and markers of corticosteroid sensitivity in asthmatic and non-asthmatic control children, for comparison with published reports in adults. Methods: AEC were obtained by nasal brushings and primary submerged cultures, and incubated in control conditions or in the presence of 10 ng/ml TNFalpha, 10-8M dexamethasone, or both. RT-PCR-based expression of FKBP51 (a steroid hormone receptor signalling regulator), NF-kB, IL-6, LIF (an IL-6 family neurotrophic cytokine), serpinB2 (which inhibits plasminogen activation and promotes fibrin deposition) and porin (a marker of mitochondrial mass) were determined. Results: 6 patients without asthma (median age 11yr; min-max: 7–13), 8 with controlled asthma (11yr, 7–13; median daily fluticasone dose = 100 μg), and 4 with uncontrolled asthma (12yr, 7–14; 1000 μg fluticasone daily) were included. Baseline expression of LIF mRNA was significantly increased in uncontrolled vs controlled asthmatic children. TNFalpha significantly increased LIF expression in uncontrolled asthma. A similar trend was observed regarding IL-6. Dexamethasone significantly upregulated FKBP51 expression in all groups but the response was blunted in asthmatic children. No significant upregulation was identified regarding NF-kB, serpinB2 and porin. Conclusion: LIF and FKBP51 expression in epithelial cells were the most interesting markers of AEC dysfunction/response to corticosteroid treatment. | |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject | Analyse comparative | |
| dc.subject | Asthme | |
| dc.subject | Enfant | |
| dc.subject | Expression des gènes | |
| dc.subject | Marqueur | |
| dc.subject.en | Bronchial asthma | |
| dc.subject.en | Children | |
| dc.subject.en | Comparative analysis | |
| dc.title.en | Nasal airway epithelial cell IL-6 and FKBP51 gene expression and steroid sensitivity in asthmatic children | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1371/journal.pone.0177051 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC] | en_US |
| dc.identifier.pubmed | 28493984 | en_US |
| bordeaux.journal | PLoS ONE | en_US |
| bordeaux.page | 1-13 | en_US |
| bordeaux.volume | 12 | en_US |
| bordeaux.hal.laboratories | NutriNeurO (Laboratoire de Nutrition et Neurobiologie Intégrée) - UMR 1286 | en_US |
| bordeaux.issue | 5 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | INRAE | en_US |
| bordeaux.team | Nutrition, mémoire et glucocorticoïdes | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.export | false | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS%20ONE&rft.date=2017&rft.volume=12&rft.issue=5&rft.spage=1-13&rft.epage=1-13&rft.eissn=1932-6203&rft.issn=1932-6203&rft.au=FAYON,%20Michael&LACOSTE-RODRIGUES,%20Aurelie&BARAT,%20Pascal&HELBLING,%20Jean%20Christophe&NACKA,%20Fabienne&rft.genre=article |
