Docosahexaenoic acid-containing choline phospholipid modulates LPS-induced neuroinflammation in vivo and in microglia in vitro
| dc.rights.license | open | en_US |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | FOURRIER, Celia | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | REMUS-BOREL, Julie | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | GREENHALGH, Andrew D. | |
| dc.contributor.author | GUICHARDANT, Michel | |
| dc.contributor.author | BERNOUD-HUBAC, Nathalie | |
| dc.contributor.author | LAGARDE, Michel | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | JOFFRE, Corinne | |
| hal.structure.identifier | Nutrition et Neurobiologie intégrée [NutriNeuro] | |
| dc.contributor.author | LAYE, Sophie
ORCID: 0000-0002-3843-1012 IDREF: 11366883X | |
| dc.date.accessioned | 2021-09-08T08:02:25Z | |
| dc.date.available | 2021-09-08T08:02:25Z | |
| dc.date.issued | 2017-08-24 | |
| dc.identifier.issn | 1742-2094 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/112148 | |
| dc.description.abstractEn | BACKGROUND: Neuroinflammatory processes are considered a double-edged sword, having both protective and detrimental effects in the brain. Microglia, the brain's resident innate immune cells, are a key component of neuroinflammatory response. There is a growing interest in developing drugs to target microglia and control neuroinflammatory processes. In this regard, docosahexaenoic acid (DHA), the brain's n-3 polyunsaturated fatty acid, is a promising molecule to regulate pro-inflammatory microglia and cytokine production. Several works reported that the bioavailability of DHA to the brain is higher when DHA is acylated to phospholipid. In this work, we analyzed the anti-inflammatory activity of DHA-phospholipid, either acetylated at the sn-1 position (AceDoPC, a stable form thought to have superior access to the brain) or acylated with palmitic acid at the sn-1 position (PC-DHA) using a lipopolysaccharide (LPS)-induced neuroinflammation model both in vitro and in vivo. METHODS: In vivo, adult C57Bl6/J mice were injected intravenously (i.v.) with either AceDoPC or PC-DHA 24 h prior to LPS (i.p.). For in vitro studies, immortalized murine microglia cells BV-2 were co-incubated with DHA forms and LPS. AceDoPC and PC-DHA effect on brain or BV-2 PUFA content was assessed by gas chromatography. LPS-induced pro-inflammatory cytokines interleukin IL-1β, IL-6, and tumor necrosis factor (TNF) α production were measured by quantitative PCR (qPCR) or multiplex. IL-6 receptors and associated signaling pathway STAT3 were assessed by FACS analysis and western-blot in vitro. RESULTS: In vivo, a single injection of AceDoPC or PC-DHA decreased LPS-induced IL-6 production in the hippocampus of mice. This effect could be linked to their direct effect on microglia, as revealed in vitro. In addition, AceDoPC or PC-DHA reduced IL-6 receptor while only AceDoPC decreased IL-6-induced STAT3 phosphorylation. CONCLUSIONS: These results highlight the potency of administered DHA-acetylated to phospholipids-to rapidly regulate LPS-induced neuroinflammatory processes through their effect on microglia. In particular, both IL-6 production and signaling are targeted by AceDoPC in microglia. | |
| dc.description.sponsorship | Metabolism in human of a structured phospholipid from marine origin and neural effect - ANR-07-PNRA-0022 | en_US |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject.en | AceDoPC | |
| dc.subject.en | Animals | |
| dc.subject.en | Choline | |
| dc.subject.en | DHA | |
| dc.subject.en | Docosahexaenoic Acids | |
| dc.subject.en | IL-1β | |
| dc.subject.en | IL-6 | |
| dc.subject.en | Inflammation | |
| dc.subject.en | Inflammation Mediators | |
| dc.subject.en | Intravenous | |
| dc.subject.en | Lipopolysaccharides | |
| dc.subject.en | Mice | |
| dc.subject.en | Microglia | |
| dc.subject.en | PC-DHA | |
| dc.subject.en | Phosphatidylcholines | |
| dc.subject.en | Phospholipids | |
| dc.subject.en | STAT3 | |
| dc.subject.en | TNFα | |
| dc.subject.en | Cell Line Transformed | |
| dc.subject.en | Mice Inbred C57BL | |
| dc.title.en | Docosahexaenoic acid-containing choline phospholipid modulates LPS-induced neuroinflammation in vivo and in microglia in vitro | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1186/s12974-017-0939-x | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC] | en_US |
| dc.identifier.pubmed | 28838312 | en_US |
| bordeaux.journal | Journal of Neuroinflammation | en_US |
| bordeaux.page | 170 | en_US |
| bordeaux.volume | 14 | en_US |
| bordeaux.hal.laboratories | NutriNeurO (Laboratoire de Nutrition et Neurobiologie Intégrée) - UMR 1286 | en_US |
| bordeaux.issue | 1 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | INRAE | en_US |
| bordeaux.team | Psychoneuroimmunologie et Nutrition: Approches expérimentales et cliniques | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.identifier.funderID | Institut National de la Recherche Agronomique | en_US |
| bordeaux.identifier.funderID | Agence Nationale de la Recherche | en_US |
| bordeaux.identifier.funderID | Conseil Régional Aquitaine | en_US |
| bordeaux.identifier.funderID | AgreenSkills | en_US |
| hal.export | false | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Neuroinflammation&rft.date=2017-08-24&rft.volume=14&rft.issue=1&rft.spage=170&rft.epage=170&rft.eissn=1742-2094&rft.issn=1742-2094&rft.au=FOURRIER,%20Celia&REMUS-BOREL,%20Julie&GREENHALGH,%20Andrew%20D.&GUICHARDANT,%20Michel&BERNOUD-HUBAC,%20Nathalie&rft.genre=article |
