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Overproduction of ascorbic acid impairs pollen fertility in tomato

hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorDESLOUS, Paul
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierMonsanto Company
dc.contributor.authorBOURNONVILLE, Céline
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDECROS, Guillaume
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorOKABE, Yoshihiro
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUXION, Jean-Philippe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorJORLY, Joana
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGADIN, Stéphanie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBRES, Cécile
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMORI, Kentaro
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFERRAND, Carine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPRIGENT, Sylvain
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorARIIZUMI, Tohru
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorEZURA, Hiroshi
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorHERNOULD, Michel
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPÉTRIACQ, Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGIBON, Yves
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBALDET, Pierre
dc.date.issued2021
dc.identifier.issn0022-0957
dc.description.abstractEnAscorbate is a major antioxidant buffer in plants, so several approaches have been developed to increase the ascorbate contents of fruits and vegetables. In this study, we combined forward genetics with mapping-by-sequencing approaches using an EMSMicro-Tom population to identify putative regulators underlying a high ascorbate phenotype in fruits. Among the ascorbate-enriched mutants, the family with the highest fruit ascorbate level (P17C5 line, up to 5 times the WT) strongly impaired flower development and produced seedless fruit. Without progeny, genetic characterization was performed by outcrossing the P17C5 line with S. Lycopersicum cv. M82. We successfully identified the mutation responsible for the high ascorbate trait in a cis-acting upstream open reading frame (uORF) that is involved in the downstream regulation of GDP-L-galactose phosphorylase (GGP). Using a specific CRISPR strategy, we generated uORF-GGP1 mutants and confirmed the ascorbate-enriched phenotype. We further investigated the impact of the ascorbate-enrichment trait in tomato plants by phenotyping the original P17C5 EMS mutant, the population of outcrossed P17C5xM82 plants, and the CRISPR-mutated line. These studies revealed that a high ascorbate content is linked to impaired floral organ architecture, particularly anthers and pollen development, thus leading to male sterility. RNAseq analysis suggests that uORF-GGP1 acts as a regulator of ascorbate synthesis that maintains redox homeostasis to allow appropriate plant development.
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.subjectPlante fruitère
dc.subjecttomate
dc.subject.enAscorbate
dc.subject.enEMS Micro-Tom mutants
dc.subject.enFertility
dc.subject.enFruits
dc.subject.enGDP-L-galactose phosphorylase
dc.subject.enPollen.
dc.title.enOverproduction of ascorbic acid impairs pollen fertility in tomato
dc.typeArticle de revue
dc.identifier.doi10.1093/jxb/erab040
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Amélioration des plantes
bordeaux.journalJournal of Experimental Botany
bordeaux.page3091-3107
bordeaux.volume72
bordeaux.issue8
bordeaux.peerReviewedoui
hal.identifierhal-03152478
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03152478v1
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