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Metallic oxide nanoparticle translocation across the human bronchial epithelial barrier

dc.contributor.authorGEORGE, Isabelle
dc.contributor.authorNAUDIN, Gregoire
dc.contributor.authorBOLAND, Sonja
dc.contributor.authorMORNET, Stephane
dc.contributor.authorCONTREMOULINS, Vincent
dc.contributor.authorBEUGNON, Karine
dc.contributor.authorMARTINON, Laurent
dc.contributor.authorLAMBERT, Olivier
dc.contributor.authorBAEZA-SQUIBAN, Armelle
dc.date.accessioned2020-09-03T08:02:10Z
dc.date.available2020-09-03T08:02:10Z
dc.date.issued2015
dc.identifier.issn2040-3364
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10979
dc.description.abstractEnInhalation is the most frequent route of unintentional exposure to nanoparticles (NPs). Our aim was to quantify the translocation of different metallic NPs across human bronchial epithelial cells and to determine the factors influencing this translocation. Calu-3 cells forming a tight epithelial barrier when grown on a porous membrane in a two compartment chamber were exposed to fluorescently labelled NPs to quantify the NP translocation. NP translocation and uptake by cells were also studied by confocal and transmission electron microscopy. Translocation was characterized according to NP size (16, 50, or 100 nm), surface charge (negative or positive SiO2), composition (SiO2 or TiO2), presence of proteins or phospholipids and in an inflammatory context. Our results showed that NPs can translocate through the Calu-3 monolayer whatever their composition (SiO2 or TiO2), but this translocation was increased for the smallest and negatively charged NPs. Translocation was not associated with an alteration of the integrity of the epithelial monolayer, suggesting a transcytosis of the internalized NPs. By modifying the NP corona, the ability of NPs to cross the epithelial barrier differed depending on their intrinsic properties, making positively charged NPs more prone to translocate. NP translocation can be amplified by using agents known to open tight junctions and to allow paracellular passage. NP translocation was also modulated when mimicking an inflammatory context frequently found in the lungs, altering the epithelial integrity and inducing transient tight junction opening. This in vitro evaluation of NP translocation could be extended to other inhaled NPs to predict their biodistribution.
dc.language.isoen
dc.title.enMetallic oxide nanoparticle translocation across the human bronchial epithelial barrier
dc.typeArticle de revue
dc.subject.halChimie/Matériaux
bordeaux.journalNanoscale
bordeaux.page4529-4544
bordeaux.volume7
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.issue10
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nanoscale&rft.date=2015&rft.volume=7&rft.issue=10&rft.spage=4529-4544&rft.epage=4529-4544&rft.eissn=2040-3364&rft.issn=2040-3364&rft.au=GEORGE,%20Isabelle&NAUDIN,%20Gregoire&BOLAND,%20Sonja&MORNET,%20Stephane&CONTREMOULINS,%20Vincent&rft.genre=article


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