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Hybrid Structure of the Type 1 Pilus of Uropathogenic Escherichia coli

dc.contributor.authorHABENSTEIN, Birgit
dc.contributor.authorLOQUET, Antoine
dc.contributor.authorHWANG, Songhwan
dc.contributor.authorGILLER, Karin
dc.contributor.authorVASA, Suresh Kumar
dc.contributor.authorBECKER, Stefan
dc.contributor.authorHABECK, Michael
dc.contributor.authorLANGE, Adam
dc.date.accessioned2020-09-03T08:02:09Z
dc.date.available2020-09-03T08:02:09Z
dc.date.issued2015
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10978
dc.description.abstractEnType 1 pili are filamentous protein assemblies on the surface of Gram-negative bacteria that mediate adhesion to host cells during the infection process. The molecular structure of type 1 pili remains elusive on the atomic scale owing to their insolubility and noncrystallinity. Herein we describe an approach for hybrid-structure determination that is based on data from solution-state NMR spectroscopy on the soluble subunit and solid-state NMR spectroscopy and STEM data on the assembled pilus. Our approach is based on iterative modeling driven by structural information extracted from different sources and provides a general tool to access pseudo atomic structures of protein assemblies with complex subunit folds. By using this methodology, we determined the local conformation of the FimA pilus subunit in the context of the assembled type 1 pilus, determined the exact helical pilus architecture, and elucidated the intermolecular interfaces contributing to pilus assembly and stability with atomic detail.
dc.language.isoen
dc.title.enHybrid Structure of the Type 1 Pilus of Uropathogenic Escherichia coli
dc.typeArticle de revue
dc.identifier.doi10.1002/anie.201505065
dc.subject.halChimie/Matériaux
bordeaux.journalAngewandte Chemie (International ed. in English)
bordeaux.page11691-5
bordeaux.volume54
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.issue40
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Angewandte%20Chemie%20(International%20ed.%20in%20English)&rft.date=2015&rft.volume=54&rft.issue=40&rft.spage=11691-5&rft.epage=11691-5&rft.au=HABENSTEIN,%20Birgit&LOQUET,%20Antoine&HWANG,%20Songhwan&GILLER,%20Karin&VASA,%20Suresh%20Kumar&rft.genre=article


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