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Production and purification of recombinant human hepcidin-25 with authentic N and C-termini

hal.structure.identifierBiotechnologie des protéines recombinantes à visée santé
dc.contributor.authorJANAKIRAMAN, Vignesh Narasimhan
hal.structure.identifierBiotechnologie des protéines recombinantes à visée santé
dc.contributor.authorCABANNE, Charlotte
dc.contributor.authorDIERYCK, Wilfrid
dc.contributor.authorHOCQUELLET, Agnes
dc.contributor.authorJOUCLA, Gilles
dc.contributor.authorLE SENECHAL, Caroline
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCHAIGNEPAIN, Stephane
dc.contributor.authorCOSTAGLIOLI, Patricia
dc.contributor.authorSANTARELLI, Xavier
hal.structure.identifierBiotechnologie des protéines recombinantes à visée santé
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
dc.contributor.authorGARBAY, Bertrand
ORCID: 0000-0001-5756-2627
IDREF: 033777551
dc.contributor.authorNOUBHANI, Abdelmajid
dc.date.accessioned2020-09-03T08:02:08Z
dc.date.available2020-09-03T08:02:08Z
dc.date.issued2015
dc.identifier.issn0168-1656
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10973
dc.description.abstractEnHepcidin was first identified as an antimicrobial peptide present in human serum and urine. It was later demonstrated that hepcidin is the long-sought hormone that regulates iron homeostasis in mammals. Recombinant human Hepcidin-25 (Hepc25) was expressed in Pichia pastoris using a modified version of the pPICZ alpha A vector. Hepc25 was then purified by a simple two-step chromatographic process to obtain 1.9 mg of soluble recombinant human Hepc25 per liter of culture at 96% purity. The sequence of Hepc25 and the presence of four disulfide bridges were confirmed by mass spectrometry analyses, and the recombinant Hepc25 exhibited antibacterial activity. This protocol of production and purification is the first step toward the production of human Hepc25 at a greater scale. (C) 2015 Elsevier B.V. All rights reserved.
dc.language.isoen
dc.title.enProduction and purification of recombinant human hepcidin-25 with authentic N and C-termini
dc.typeArticle de revue
dc.subject.halChimie/Matériaux
bordeaux.journalJournal of Biotechnology
bordeaux.page89-92
bordeaux.volume195
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
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