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Slow potentials encode intercellular coupling and insulin demand in pancreatic beta cells

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dc.relation.isnodouble321e18cf-ef58-4b90-bad3-d6c147b73f34*
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLEBRETON, Fanny
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorPIROG, Antoine
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBELOUAH, Isma
dc.contributor.authorBOSCO, Domenico
dc.contributor.authorBERNEY, Thierry
dc.contributor.authorMEDA, Paolo
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorBORNAT, Yannick
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCATARGI, Bogdan
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorRENAUD, Sylvie
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorRAOUX, Matthieu
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLANG, Jochen
dc.date.accessioned2020-09-03T08:02:05Z
dc.date.available2020-09-03T08:02:05Z
dc.date.issued2015
dc.identifier.issn0012-186x
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10962
dc.description.abstractEnAIMS/HYPOTHESIS: Ion fluxes constitute a major integrative signal in beta cells that leads to insulin secretion and regulation of gene expression. Understanding these electrical signals is important for deciphering the endogenous algorithms used by islets to attain homeostasis and for the design of new sensors for monitoring beta cell function. METHODS: Mouse and human islets were cultured on multielectrode arrays (MEAs) for 3-13 days. Extracellular electrical activities received on each electrode were continuously amplified and recorded for offline characterisation. RESULTS: Differential band-pass filtering of MEA recordings of mouse islets showed two extracellular voltage waveforms: action potentials (lasting 40-60 ms) and very robust slow potentials (SPs, lasting 800-1,500 ms), the latter of which have not been described previously. The frequency of SPs directly correlated with glucose concentration, peaked at 10 mmol/l glucose and was further augmented by picomolar concentrations of glucagon-like peptide-1. SPs required the closure of ATP-dependent potassium channels as they were induced by glucose or glibenclamide but were not elicited by KCl-induced depolarisation. Pharmacological tools and the use of beta cell specific knockout mice showed that SPs reflected cell coupling via connexin 36. Moreover, increasing and decreasing glucose ramps showed hysteresis with reduced glucose sensitivity during the decreasing phase. SPs were also observed in human islets and could be continuously recorded over 24 h. CONCLUSIONS/INTERPRETATION: This novel electrical signature reflects the syncytial function of the islets and is specific to beta cells. Moreover, the observed hysteresis provides evidence for an endogenous algorithm naturally present in islets to protect against hypoglycaemia.
dc.language.isoen
dc.title.enSlow potentials encode intercellular coupling and insulin demand in pancreatic beta cells
dc.title.alternativeDiabetologia
dc.typeArticle de revue
dc.identifier.doi10.1007/s00125-015-3558-z
dc.subject.halChimie/Matériaux
bordeaux.journalDiabetologia
bordeaux.page1291-1299
bordeaux.volume58
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.issue6
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
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