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High-speed centrifugation induces aggregation of extracellular vesicles

dc.relation.isnodouble15fd8650-7930-4567-9d12-5036f749ec35*
dc.relation.isnodouble0949fc43-15c4-4569-a0d0-d62838082e2e*
dc.contributor.authorLINARES, R.
dc.contributor.authorTAN, S.
dc.contributor.authorGOUNOU, Celine
dc.contributor.authorARRAUD, N.
dc.contributor.authorBRISSON, Alain
dc.date.accessioned2020-09-03T08:02:03Z
dc.date.available2020-09-03T08:02:03Z
dc.date.issued2015
dc.identifier.issn2001-3078
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10959
dc.description.abstractEnPlasma and other body fluids contain cell-derived extracellular vesicles (EVs), which participate in physiopathological processes and have potential biomedical applications. In order to isolate, concentrate and purify EVs, high-speed centrifugation is often used. We show here, using electron microscopy, receptor-specific gold labelling and flow cytometry, that high-speed centrifugation induces the formation of EV aggregates composed of a mixture of EVs of various phenotypes and morphologies. The presence of aggregates made of EVs of different phenotypes may lead to erroneous interpretation concerning the existence of EVs harbouring surface antigens from different cell origins.
dc.language.isoen
dc.title.enHigh-speed centrifugation induces aggregation of extracellular vesicles
dc.title.alternativeJournal of extracellular vesicles
dc.typeArticle de revue
dc.identifier.doi10.3402/jev.v4.29509
dc.subject.halChimie/Matériaux
bordeaux.journalJ Extracell Vesicles
bordeaux.page29509
bordeaux.volume4
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
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