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A comprehensive evaluation of mixed mode interactions of HEA and PPA HyperCel™ chromatographic media

dc.contributor.authorPEZZINI, J.
dc.contributor.authorCABANNE, Charlotte
dc.contributor.authorGANTIER, R.
dc.contributor.authorJANAKIRAMAN, V. N.
dc.contributor.authorSANTARELLI, X.
dc.date.accessioned2020-09-03T08:01:58Z
dc.date.available2020-09-03T08:01:58Z
dc.date.issued2015
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10939
dc.description.abstractEnMixed mode or multimodal chromatography has been developed for rational use of multiple interactions in a controlled manner, in contrast to non-specific interactions. Indeed, as the term "mixed mode" suggests, these resins allow different types of interactions within a single chromatographic medium. In this paper, HEA HyperCel™, PPA HyperCel™ mixed-mode chromatographic media have been studied. These mixed-mode sorbents typically involve hydrophobic pseudo-affinity interactions for binding and essentially ionic interactions (charge repulsion) for elution. We identified and characterized these different interactions in chromatographic experiments by exploiting specific properties of proteins using protein standards and complex mixtures. We highlighted the major intervention of at least two types of interactions in these media: hydrophobic and electrostatic interactions. We observed the behaviour of these resins at different pH, ionic strength, with different salts and buffers types and in the presence of different organic compounds. © 2014 Elsevier B.V.
dc.language.isoen
dc.title.enA comprehensive evaluation of mixed mode interactions of HEA and PPA HyperCel™ chromatographic media
dc.typeArticle de revue
dc.identifier.doi10.1016/j.jchromb.2014.11.020
dc.subject.halChimie/Matériaux
bordeaux.journalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
bordeaux.page68-77
bordeaux.volume976-977
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
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