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dc.rights.licenseopenen_US
dc.contributor.authorLAGARDE, A.
dc.contributor.authorMILLOT, M.
dc.contributor.authorPINON, A.
dc.contributor.authorLIAGRE, B.
dc.contributor.authorGIRARDOT, M.
dc.contributor.authorIMBERT, C.
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorOUK, T. S.
dc.contributor.authorJARGEAT, P.
dc.contributor.authorMAMBU, L.
dc.date.accessioned2021-07-16T09:46:07Z
dc.date.available2021-07-16T09:46:07Z
dc.date.issued2019
dc.identifier.issn1364-5072en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/109274
dc.description.abstractEnAims The objective of this study was to explore the diversity of endolichenic fungi from Nephroma laevigatum and to investigate their antiproliferative and antibiofilm potential. Methods and Results Forty-six isolates were obtained and identified by DNA barcoding. They belonged to genera Nemania, Daldinia, Peziza and Coniochaeta. Six strains belonging to the most represented species were selected and tested for their antiproliferative and antibiofilm activities. Extracts were analysed by reversed-phase HPLC. Activities against fungal and bacterial biofilm were evaluated using tetrazolium salt (XTT) assay and crystal violet assay respectively. Antiproliferative responses of extracts were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptosis induction by two extracts was observed in two cell lines (HT-29 and PC-3) via morphological changes, pro-apoptotic and anti-apoptotic proteins analysis (Western blotting) and DNA fragmentation. Four extracts displayed activities against Candida albicans biofilm with IC50 values ranging from 25 to 200 mu g ml(-1). All extracts were inactive against Staphylococcus aureus and Pseudomonas aeruginosa biofilms. The most active isolates against human colorectal (HT-29 and HCT116) and prostate (PC-3 and DU145) cancer cell lines were Nemania serpens (NL08) and Nemania aenea var. aureolatum (NL38) with IC50 values ranging from 13 to 39 mu g ml(-1). These extracts induced an apoptotic process through activation of caspases 8 and 3, poly(ADP-ribose) polymerase cleavage and DNA fragmentation. Conclusions Selected crude fungal extracts have antiproliferative and antibiofilm activities. Data suggest that this antipoliferative effect is due to apoptosis process. This is the first report showing the effects of endolichenic fungi from N. laevigatum. Significance and Impact of the Study This study highlights the therapeutic potential of endolichenic fungi metabolites as sources for drug discovery programmes.
dc.language.isoENen_US
dc.subject.enapoptosis
dc.subject.encancer cell lines
dc.subject.enCandida albicans
dc.subject.enDNA barcoding
dc.subject.enNemania sp.
dc.subject.enSordariomycetes
dc.title.enAntiproliferative and antibiofilm potentials of endolichenic fungi associated with the lichen Nephroma laevigatum
dc.typeArticle de revueen_US
dc.identifier.doi10.1111/jam.14188en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalJournal of Applied Microbiologyen_US
bordeaux.page1044-1058en_US
bordeaux.volume126en_US
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248en_US
bordeaux.issue4en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02295501
hal.exportfalse
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