Afficher la notice abrégée

Time-lapse scanning surface plasmon microscopy of living adherent cells with a radially polarized beam

dc.contributor.authorBERGUIGA, L.
dc.contributor.authorSTREPPA, L.
dc.contributor.authorBOYER-PROVERA, Elise
dc.contributor.authorMARTINEZ-TORRES, C.
dc.contributor.authorSCHAEFFER, L.
dc.contributor.authorELEZGARAY, Juan
dc.contributor.authorARNEODO, A.
dc.contributor.authorARGOUL, F.
dc.date.accessioned2020-09-03T07:56:31Z
dc.date.available2020-09-03T07:56:31Z
dc.date.issued2016
dc.identifier.issn1559-128X
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10915
dc.description.abstractEnWe report on a fibered high-resolution scanning surface plasmon microscope for long term imaging of living adherent cells. The coupling of a high numerical aperture objective lens and a fibered heterodyne interferometer enhances both the sensitivity and the long term stability of this microscope, allowing for time-lapse recording over several days. The diffraction limit is reached with a radially polarized illumination beam. Adherence and motility of living C2C12 myoblast cells are followed for 50 h, revealing that the dynamics of these cells change after 10 h. This plasmon enhanced evanescent wave microscopy is particularly suited for investigating cell adhesion, since it can not only be performed without staining of the sample but it can also capture in real time the exchange of extracellular matrix elements between the substrate and the cells. (C) 2016 Optical Society of America
dc.language.isoen
dc.title.enTime-lapse scanning surface plasmon microscopy of living adherent cells with a radially polarized beam
dc.typeArticle de revue
dc.identifier.doi10.1364/ao.55.001216
dc.subject.halChimie/Matériaux
bordeaux.journalApplied Optics
bordeaux.page1216-1227
bordeaux.volume55
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.issue6
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Applied%20Optics&rft.date=2016&rft.volume=55&rft.issue=6&rft.spage=1216-1227&rft.epage=1216-1227&rft.eissn=1559-128X&rft.issn=1559-128X&rft.au=BERGUIGA,%20L.&STREPPA,%20L.&BOYER-PROVERA,%20Elise&MARTINEZ-TORRES,%20C.&SCHAEFFER,%20L.&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée