Hevea brasiliensis prohevein possesses a conserved C-terminal domain with amyloid-like properties in vitro
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| dc.relation.isnodouble | 126070b1-3bea-4167-83db-9357a0e84f98 | * |
| dc.relation.isnodouble | b01b560f-b3c7-4d01-9f2f-5744f4929fdd | * |
| dc.contributor.author | BERTHELOT, K. | |
| dc.contributor.author | LECOMTE, S. | |
| dc.contributor.author | COULARY-SALIN, B. | |
| dc.contributor.author | BENTALEB, A. | |
| dc.contributor.author | PERUCH, F. | |
| dc.date.accessioned | 2020-09-03T07:56:30Z | |
| dc.date.available | 2020-09-03T07:56:30Z | |
| dc.date.issued | 2016 | |
| dc.identifier.issn | 0006-3002 (Print) 0006-3002 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/10913 | |
| dc.description.abstractEn | Prohevein is a wound-induced protein and a main allergen from latex of Hevea brasiliensis (rubber tree). This 187 amino-acid protein is cleaved in two fragments: a N-terminal 43 amino-acids called hevein, a lectin bearing a chitin-binding motif with antifungal properties and a C-terminal domain (C-ter) far less characterized. We provide here new insights on the characteristics of prohevein, hevein and C-terminal domain. Using complementary biochemical (ThT/CR/chitin binding, agglutination) and structural (modeling, ATR-FTIR, TEM, WAXS) approaches, we show that this domain clearly displays all the characteristics of an amyloid-like proteins in vitro, that could confer agglutination activity in synergy with its chitin-binding activity. Additionally, this C-ter domain is highly conserved and present in numerous plant prohevein-like proteins or pathogenesis-related (PR and WIN) proteins. This could be the hallmark of the eventual presence of proteins with amyloid properties in plants, that could potentially play a role in defense through aggregation properties. | |
| dc.language.iso | en | |
| dc.title.en | Hevea brasiliensis prohevein possesses a conserved C-terminal domain with amyloid-like properties in vitro | |
| dc.title.alternative | Biochimica et biophysica acta | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1016/j.bbapap.2016.01.006 | |
| dc.subject.hal | Chimie/Matériaux | |
| bordeaux.journal | Biochim Biophys Acta | |
| bordeaux.page | 388-399 | |
| bordeaux.volume | 1864 | |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248 | * |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248) | |
| bordeaux.issue | 4 | |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.institution | Bordeaux INP | |
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