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Real time monitoring of membrane GPCR reconstitution by plasmon waveguide resonance: on the role of lipids

dc.contributor.authorCALMET, Pierre
dc.contributor.authorDE MARIA, Monica
dc.contributor.authorHARTE, Etienne
dc.contributor.authorLAMB, Daniel
dc.contributor.authorSERRANO-VEGA, Maria
dc.contributor.authorJAZAYERI, Ali
dc.contributor.authorTSCHAMMER, Nuska
dc.contributor.authorALVES, Isabel
dc.date.accessioned2020-09-03T07:56:25Z
dc.date.available2020-09-03T07:56:25Z
dc.date.issued2016
dc.identifier.issn2045-2322
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10897
dc.description.abstractEnG-protein coupled receptors (GPCRs) are important therapeutic targets since more than 40% of the drugs on the market exert their action through these proteins. To decipher the molecular mechanisms of activation and signaling, GPCRs often need to be isolated and reconstituted from a detergent-solubilized state into a well-defined and controllable lipid model system. Several methods exist to reconstitute membrane proteins in lipid systems but usually the reconstitution success is tested at the end of the experiment and often by an additional and indirect method. Irrespective of the method used, the reconstitution process is often an intractable and time-consuming trial-and-error procedure. Herein, we present a method that allows directly monitoring the reconstitution of GPCRs in model planar lipid membranes. Plasmon waveguide resonance (PWR) allows following GPCR lipid reconstitution process without any labeling and with high sensitivity. Additionally, the method is ideal to probe the lipid effect on receptor ligand binding as demonstrated by antagonist binding to the chemokine CCR5 receptor.
dc.language.isoen
dc.title.enReal time monitoring of membrane GPCR reconstitution by plasmon waveguide resonance: on the role of lipids
dc.typeArticle de revue
dc.identifier.doi10.1038/srep36181
dc.subject.halChimie/Matériaux
bordeaux.journalScientific Reports
bordeaux.volume6
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific%20Reports&rft.date=2016&rft.volume=6&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=CALMET,%20Pierre&DE%20MARIA,%20Monica&HARTE,%20Etienne&LAMB,%20Daniel&SERRANO-VEGA,%20Maria&rft.genre=article


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