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Biophysical analysis of the plant-specific GIPC sphingolipids reveals multiple modes of membrane regulation

dc.rights.licenseopenen_US
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorMAMODE CASSIM, Adiilah
dc.contributor.authorNAVON, Yotam
dc.contributor.authorGAO, Yu
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorDECOSSAS, Marion
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorFOUILLEN, Laetitia
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorGRELARD, Axelle
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorNAGANO, Minoru
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLAMBERT, Olivier
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorBAHAMMOU, Delphine
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorVAN DELFT, Pierre
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorMANETA-PEYRET, Lilly
dc.contributor.authorSIMON-PLAS, Francoise
dc.contributor.authorHEUX, Laurent
dc.contributor.authorJEAN, Bruno
dc.contributor.authorFRAGNETO, Giovanna
dc.contributor.authorMORTIMER, Jenny C.
dc.contributor.authorDELEU, Magali
dc.contributor.authorLINS, Laurence
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorMONGRAND, Sébastien
dc.date.accessioned2021-07-09T15:05:55Z
dc.date.available2021-07-09T15:05:55Z
dc.date.issued2021
dc.identifier.issn1083-351Xen_US
dc.identifier.otherhttps://www.jbc.org/action/showPdf?pii=S0021-9258%2821%2900382-3#%FE%FF%00a%00p%00p%00s%00e%00c%001en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/106505
dc.description.abstractEnThe plant plasma membrane (PM) is an essential barrier between the cell and the external environment, controlling signal perception and transmission. It consists of an asymmetrical lipid bilayer made up of three different lipid classes: sphingolipids, sterols and phospholipids. The Glycosyl Inositol Phosphoryl Ceramides (GIPCs), representing up to 40% of total sphingolipids, are assumed to be almost exclusively in the outer leaflet of the PM. However, their biological role and properties are poorly defined. In this study, we investigated the role of GIPCs in membrane organization. Since GIPCs are not commercially available, we developed a protocol to extract and isolate GIPC-enriched fractions from eudicots (cauliflower and tobacco) and monocots (leek and rice). Lipidomic analysis confirmed the presence of trihydroxylated long chain bases and 2-hydroxylated very long chain fatty acids up to 26 carbon atoms. The glycan head groups of the GIPCs from monocots and dicots were analyzed by GC-MS, revealing different sugar moieties. Multiple biophysics tools, namely Langmuir monolayer, zeta-Potential, light scattering, neutron reflectivity, solid state 2H-NMR and molecular modelling, were used to investigate the physical properties of the GIPCs, as well as their interaction with free and conjugated phytosterols. We showed that GIPCs increase the thickness and electronegativity of model membranes, interact differentially with the different phytosterols species and regulate the gel-to-fluid phase transition during temperature variations. These results unveil the multiple roles played by GIPCs in the plant plasma membrane.
dc.description.sponsorshipVers un modèle intégratif de la bicouche lipidique de la membrane plasmique végétale - ANR-19-CE20-0016en_US
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enCryo-EM
dc.subject.enSolid state NMR
dc.subject.enNeutron reflectivity
dc.subject.enLangmuir monolayer
dc.subject.enζ-Potential
dc.subject.enModelling
dc.subject.enPlant
dc.subject.enPlasma membrane
dc.subject.enSphingolipids
dc.subject.enGIPC
dc.subject.enPhytosterol
dc.subject.enPurification
dc.subject.enLipidomics
dc.title.enBiophysical analysis of the plant-specific GIPC sphingolipids reveals multiple modes of membrane regulation
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.jbc.2021.100602en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalThe Journal of biological chemistryen_US
bordeaux.page100602-100602en_US
bordeaux.volume296en_US
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-03189252
hal.exportfalse
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