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Dynamic Uni- and Multicellular Patterns Encode Biphasic Activity in Pancreatic Islets

dc.rights.licenseopenen_US
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorJAFFREDO, Manon
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBERTIN, Eleonore
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorPIROG, Antoine
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorPUGINIER, Emilie
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorGAITAN, Julien
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorOUCHERIF, Sandra
dc.contributor.authorLEBRETON, Fanny
dc.contributor.authorBOSCO, Domenico
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCATARGI, Bogdan
dc.contributor.authorCATTAERT, Daniel
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorRENAUD, Sylvie
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLANG, Jochen
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorRAOUX, Matthieu
dc.date.accessioned2021-07-08T14:25:06Z
dc.date.available2021-07-08T14:25:06Z
dc.date.issued2021
dc.identifier.issn0012-1797en_US
dc.identifier.otherhttps://doi.org/10.2337/figshare.13562354en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/106478
dc.description.abstractEnBiphasic secretion is an autonomous feature of many endocrine micro-organs to fulfill physiological demands. The biphasic activity of islet beta-cells maintains glucose homeostasis and is altered in type 2 diabetes. Nevertheless, underlying cellular or multicellular functional organizations are only partially understood. High-resolution noninvasive multielectrode array recordings permit simultaneous analysis of recruitment, of single-cell, and of coupling activity within entire islets in long-time experiments. Using this unbiased approach, we addressed the organizational modes of both first and second phase in mouse and human islets under physiological and pathophysiological conditions. Our data provide a new uni- and multicellular model of islet beta-cell activation: during the first phase, small but highly active beta-cell clusters are dominant, whereas during the second phase, electrical coupling generates large functional clusters via multicellular slow potentials to favor an economic sustained activity. Postprandial levels of glucagon-like peptide 1 favor coupling only in the second phase, whereas aging and glucotoxicity alter coupled activity in both phases. In summary, biphasic activity is encoded upstream of vesicle pools at the micro-organ level by multicellular electrical signals and their dynamic synchronization between beta-cells. The profound alteration of the electrical organization of islets in pathophysiological conditions may contribute to functional deficits in type 2 diabetes.
dc.language.isoENen_US
dc.title.enDynamic Uni- and Multicellular Patterns Encode Biphasic Activity in Pancreatic Islets
dc.typeArticle de revueen_US
dc.identifier.doi10.2337/db20-0214en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalDiabetesen_US
bordeaux.page878-888en_US
bordeaux.volume70en_US
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248en_US
bordeaux.issue4en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-03282033
hal.version1
hal.date.transferred2021-07-08T14:25:11Z
hal.exporttrue
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