Membrane Repair Assay for Human Skeletal Muscle Cells
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | CARMEILLE, Romain | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | CROISSANT, Coralie | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | BOUVET, Flora | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | BOUTER, Anthony | |
| dc.date.accessioned | 2020-07-09T14:17:07Z | |
| dc.date.available | 2020-07-09T14:17:07Z | |
| dc.date.issued | 2017 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/10347 | |
| dc.description.abstractEn | The characterization of the membrane repair machinery in human skeletal muscle has become crucial, since it has been shown that some muscular dystrophies result from a defect of this fundamental physiological process. Deciphering membrane repair mechanism requires the development of methodologies allowing studying the response of skeletal muscle cells to sarcolemma damage and identifying candidate proteins playing a role in the membrane repair machinery. Here, we describe a protocol that is based on the creation of cell membrane disruption by infrared laser irradiation in human myotubes. Membrane disruption and repair are assayed by monitoring the incorporation into myotubes of the membrane probe FM1-43. This methodology has recently enabled us to show that Annexin-A5 is required for membrane repair in human skeletal muscle cells (Carmeille et al., Biochim Biophys Acta 1863:2267-2279, 2016). | |
| dc.title.en | Membrane Repair Assay for Human Skeletal Muscle Cells | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1007/978-1-4939-7283-8_14 | |
| dc.subject.hal | Chimie/Matériaux | |
| bordeaux.journal | Methods in molecular biology (Clifton, N.J.) | |
| bordeaux.page | 195-207 | |
| bordeaux.volume | 1668 | |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248 | * |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248) | |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.institution | Bordeaux INP | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Methods%20in%20molecular%20biology%20(Clifton,%20N.J.)&rft.date=2017&rft.volume=1668&rft.spage=195-207&rft.epage=195-207&rft.au=CARMEILLE,%20Romain&CROISSANT,%20Coralie&BOUVET,%20Flora&BOUTER,%20Anthony&rft.genre=article |
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