Rescue of Hearing by Gene Delivery to Inner-Ear Hair Cells Using Exosome-Associated AAV
| dc.contributor.author | GYORGY, Bence | |
| dc.contributor.author | SAGE, Cyrille | |
| dc.contributor.author | INDZHYKULIAN, Artur A. | |
| dc.contributor.author | SCHEFFER, Deborah I. | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | BRISSON, Alain | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | TAN, Sisareuth | |
| dc.contributor.author | WU, Xudong | |
| dc.contributor.author | VOLAK, Adrienn | |
| dc.contributor.author | MU, Dakai | |
| dc.contributor.author | TAMVAKOLOGOS, Panos I. | |
| dc.contributor.author | LI, Yaqiao | |
| dc.contributor.author | FITZPATRICK, Zachary | |
| dc.contributor.author | ERICSSON, Maria | |
| dc.contributor.author | BREAKEFIELD, Xandra O. | |
| dc.contributor.author | COREY, David P. | |
| dc.contributor.author | MAGUIRE, Casey A. | |
| dc.date.accessioned | 2020-07-09T14:16:53Z | |
| dc.date.available | 2020-07-09T14:16:53Z | |
| dc.date.issued | 2017-02 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/10319 | |
| dc.description.abstractEn | Adeno-associated virus (AAV) is a safe and effective vector for gene therapy for retinal disorders. Gene therapy for hearing disorders is not as advanced, in part because gene delivery to sensory hair cells of the inner ear is inefficient. Although AAV transduces the inner hair cells of the mouse cochlea, outer hair cells remain refractory to transduction. Here, we demonstrate that a vector, exosome-associated AAV (exo-AAV), is a potent carrier of transgenes to all inner ear hair cells. Exo-AAV1-GFP is more efficient than conventional AAV1-GFP, both in mouse cochlear explants invitro and with direct cochlear injection invivo. Exo-AAV shows no toxicity invivo, as assayed by tests of auditory and vestibular function. Finally, exo-AAV1 gene therapy partially rescues hearing in a mouse model of hereditary deafness (lipoma HMGIC fusion partner-like 5/tetraspan membrane protein of hair cell stereocilia [Lhfpl5/Tmhs-/-]). Exo-AAV is a powerful gene delivery system for hair cell research and may be useful for gene therapy for deafness. | |
| dc.title.en | Rescue of Hearing by Gene Delivery to Inner-Ear Hair Cells Using Exosome-Associated AAV | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1016/j.ymthe.2016.12.010 | |
| dc.subject.hal | Chimie/Matériaux | |
| bordeaux.journal | Molecular therapy : the journal of the American Society of Gene Therapy | |
| bordeaux.page | 379-391 | |
| bordeaux.volume | 25 | |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248 | * |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248) | |
| bordeaux.issue | 2 | |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.institution | Bordeaux INP | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular%20therapy%20:%20the%20journal%20of%20the%20American%20Society%20of%20Gene%20Therapy&rft.date=2017-02&rft.volume=25&rft.issue=2&rft.spage=379-391&rft.epage=379-391&rft.au=GYORGY,%20Bence&SAGE,%20Cyrille&INDZHYKULIAN,%20Artur%20A.&SCHEFFER,%20Deborah%20I.&BRISSON,%20Alain&rft.genre=article |
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