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Exploiting Benzophenone Photoreactivity To Probe the Phospholipid Environment and Insertion Depth of the Cell-Penetrating Peptide Penetratin in Model Membranes

dc.contributor.authorJIAO, C. Y.
dc.contributor.authorSACHON, E.
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorALVES, Isabel
dc.contributor.authorCHASSAING, G.
dc.contributor.authorBOLBACH, G.
dc.contributor.authorSAGAN, S.
dc.date.accessioned2020-07-09T14:16:49Z
dc.date.available2020-07-09T14:16:49Z
dc.date.issued2017-05
dc.identifier.issn1433-7851
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10309
dc.description.abstractEnPenetratin (RQIKIWFQNRRMKWKK) enters cells by different mechanisms, including membrane translocation, thus implying that the peptide interacts with the lipid bilayer. Penetratin also crosses the membrane of artificial vesicles, depending on their phospholipid content. To evaluate the phospholipid preference of penetratin, as the first step of translocation, we exploited the benzophenone triplet kinetics of hydrogen abstraction, which is slower for secondary than for allylic hydrogen atoms. By using multilamellar vesicles of varying phospholipid content, we identified and characterized the cross-linked products by MALDI-TOF mass spectrometry. Penetratin showed a preference for negatively charged (vs. zwitterionic) polar heads, and for unsaturated (vs. saturated) and short (vs. long) saturated phospholipids. Our study highlights the potential of using benzophenone to probe the environment and insertion depth of membranotropic peptides in membranes.
dc.title.enExploiting Benzophenone Photoreactivity To Probe the Phospholipid Environment and Insertion Depth of the Cell-Penetrating Peptide Penetratin in Model Membranes
dc.title.alternativeAngewandte Chemie (International ed. in English)
dc.typeArticle de revue
dc.identifier.doi10.1002/anie.201703465
dc.subject.halChimie/Matériaux
bordeaux.journalAngew Chem Int Ed Engl
bordeaux.page8226-8230
bordeaux.volume56
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.issue28
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Angew%20Chem%20Int%20Ed%20Engl&rft.date=2017-05&rft.volume=56&rft.issue=28&rft.spage=8226-8230&rft.epage=8226-8230&rft.eissn=1433-7851&rft.issn=1433-7851&rft.au=JIAO,%20C.%20Y.&SACHON,%20E.&ALVES,%20Isabel&CHASSAING,%20G.&BOLBACH,%20G.&rft.genre=article


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