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Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy

hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLINARES, Romain
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorTAN, Sisareuth
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorGOUNOU, Celine
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBRISSON, Alain
dc.date.accessioned2020-07-09T14:16:45Z
dc.date.available2020-07-09T14:16:45Z
dc.date.issued2017
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10295
dc.description.abstractEnExtracellular vesicles (EVs) are cell-derived vesicles that are present in blood and other body fluids. EVs raise major interest for their diverse physiopathological roles and their potential biomedical applications. However, the characterization and quantification of EVs constitute major challenges, mainly due to their small size and the lack of methods adapted for their study. Electron microscopy has made significant contributions to the EV field since their initial discovery. Here, we describe the use of two transmission electron microscopy (TEM) techniques for imaging and quantifying EVs. Cryo-TEM combined with receptor-specific gold labeling is applied to reveal the morphology, size, and phenotype of EVs, while their enumeration is achieved after high-speed sedimentation on EM grids.
dc.title.enImaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy
dc.typeArticle de revue
dc.identifier.doi10.1007/978-1-4939-6728-5_4
dc.subject.halChimie/Matériaux
bordeaux.journalMethods in molecular biology (Clifton, N.J.)
bordeaux.page43-54
bordeaux.volume1545
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Methods%20in%20molecular%20biology%20(Clifton,%20N.J.)&rft.date=2017&rft.volume=1545&rft.spage=43-54&rft.epage=43-54&rft.au=LINARES,%20Romain&TAN,%20Sisareuth&GOUNOU,%20Celine&BRISSON,%20Alain&rft.genre=article


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