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Evaluation of the GenoType NTM-DR assay performance for the identification and molecular detection of antibiotic resistance in Mycobacterium abscessus complex

dc.rights.licenseopenen_US
dc.contributor.authorBOUZINBI, N.
hal.structure.identifierBordeaux population health [BPH]
dc.contributor.authorMARCY, Olivier
dc.contributor.authorBERTOLOTTI, T.
dc.contributor.authorCHIRON, R.
dc.contributor.authorBEMER, P.
dc.contributor.authorPESTEL-CARON, M.
dc.contributor.authorPEUCHANT, O.
dc.contributor.authorGUET-REVILLET, H.
dc.contributor.authorFANGOUS, M. S.
dc.contributor.authorHÉRY-ARNAUD, G.
dc.contributor.authorOUEDRAOGO, A. S.
dc.contributor.authorBAÑULS, A. L.
dc.contributor.authorGODREUIL, S.
dc.date.accessioned2021-01-18T12:37:17Z
dc.date.available2021-01-18T12:37:17Z
dc.date.issued2020
dc.identifier.issn1932-6203en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/25817
dc.description.abstractEnThe first objective of this study was to determine the GenoType NTM-DR assay performance for subspecies identification in Mycobacterium abscessus complex isolates. The second objective was to evaluate the GenoType NTM-DR assay ability to detect clarithromycin and amikacin resistance in M. abscessus complex isolates compared with drug susceptibility testing (DST) and PCR sequencing of the erm(41), rrl and rrs genes. The concordance between the GenoType NTM-DR and MLST results concerning subspecies identification was 100%. The wild type and mutated alleles of the rrl and rrs genes were detected by the GenoType NTM-DR assay and PCR sequencing with 100% (115/115) agreement. Similarly, 100% concordance between GenoType NTM-DR and DST was observed for clarithromycin and amikacin testing. Sensitivity for the detection of clarithromycin and amikacin resistance was 100%. The GenoType NTM-DR assay provides a robust and complementary tool to the gold standard methods (MLST and broth microdilution) for subspecies identification and drug resistance detection.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subjectIDLIC
dc.title.enEvaluation of the GenoType NTM-DR assay performance for the identification and molecular detection of antibiotic resistance in Mycobacterium abscessus complex
dc.title.alternativePLoS Oneen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1371/journal.pone.0239146en_US
dc.subject.halSciences du Vivant [q-bio]/Santé publique et épidémiologieen_US
dc.identifier.pubmed32976521en_US
bordeaux.journalPLoS ONEen_US
bordeaux.pagee0239146en_US
bordeaux.volume15en_US
bordeaux.hal.laboratoriesBordeaux Population Health Research Center (BPH) - U1219en_US
bordeaux.issue9en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.teamIDLICen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.exportfalse
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