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Impact of in vitro exposure to 5G-modulated 3.5 GHz fields on oxidative stress and DNA repair in skin cells

dc.rights.licenseopenen_US
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorHAIDAR, Jana
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorNABOS, Patricia
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorORLACCHIO, Rosa
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorHURTIER, Annabelle
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorDE GANNES, Florence Poulletier
dc.contributor.authorRAMBERT, Jérome
dc.contributor.authorCARIO-ANDRÉ, Muriel
dc.contributor.authorMOISAN, Francois
IDREF: 140488812
dc.contributor.authorREZVANI, Hamid-Reza
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorLAGROYE, Isabelle
dc.contributor.authorLEVEQUE, Philippe
dc.contributor.authorARNAUD-CORMOS, Delia
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorPERCHERANCIER, Yann
dc.date.accessioned2025-12-01T08:40:14Z
dc.date.available2025-12-01T08:40:14Z
dc.date.issued2025-08-25
dc.identifier.issn2045-2322en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/207954
dc.description.abstractEnAbstract The rapid deployment of fifth-generation (5G) wireless networks has raised societal concerns regarding potential biological effects, particularly on human skin, due to the use of higher carrier frequencies that penetrate tissue less deeply. Consequently, whether 5G-modulated radiofrequency (RF) electromagnetic fields (EMFs) at 3.5 GHz affect oxidative stress and DNA repair in skin cells remains an open question. Using genetically encoded Bioluminescence Resonance Energy Transfer (BRET)-based biosensors targeted to the cytoplasm and mitochondria, we assessed whether exposure of human fibroblasts to 5G RF-EMF at specific absorption rates (SAR) of 0.08 and 4 W/kg for 24 h could alter basal reactive oxygen species (ROS) levels or potentiate the effects of known ROS inducers, including H₂O₂, Kp372-1, and Antimycin A. We also evaluated whether pre-exposure to 5G RF-EMF could induce an adaptive response (AR), by modulating ROS production following a subsequent challenge with arsenic trioxide (As₂O₃). Additionally, we investigated the impact of combined RF-EMF and ultraviolet-B (UV-B) exposure on the formation and repair of cyclobutane pyrimidine dimer (CPD) lesions in HaCaT keratinocytes. Our results showed no significant effect of 5G RF-EMF exposure, either alone or in combination with chemical ROS inducers, on oxidative stress markers in either compartment. Likewise, RF-EMF exposure did not induce an adaptive response to oxidative challenge, nor did it alter the kinetics or the efficiency of CPD repair by the nucleotide excision repair (NER) pathway. These findings support the conclusion that the exposure to 5G RF-EMF at 3.5 GHz up to 4 W/kg does not induce oxidative stress or impair DNA repair efficiency in human skin cells, within the experimental conditions tested.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subjectBioluminescence resonance energy transfer (BRET)
dc.subjectCellular stress response
dc.subjectCyclobutane pyrimidine dimer (CPD)
dc.subjectDNA damage
dc.subjectFifth generation (5G)
dc.subjectNucleotide excision repair (NER)
dc.subjectOxidative stress
dc.subjectRadiofrequency electromagnetic fields (RF-EMF)
dc.subjectReactive oxygen species (ROS)
dc.title.enImpact of in vitro exposure to 5G-modulated 3.5 GHz fields on oxidative stress and DNA repair in skin cells
dc.typeArticle de revueen_US
dc.identifier.doi10.1038/s41598-025-15090-wen_US
dc.subject.halSciences de l'ingénieur [physics]/Traitement du signal et de l'imageen_US
dc.subject.halSciences de l'ingénieur [physics]en_US
dc.description.sponsorshipEuropeExposure To electromAgnetic fIelds and plaNetary healthen_US
bordeaux.journalScientific Reportsen_US
bordeaux.volume15en_US
bordeaux.hal.laboratoriesIMS : Laboratoire de l'Intégration du Matériau au Système - UMR 5218en_US
bordeaux.issue1en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.teamBIOELECTRONICS - SANEen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcecrossref
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcecrossref
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific%20Reports&rft.date=2025-08-25&rft.volume=15&rft.issue=1&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=HAIDAR,%20Jana&NABOS,%20Patricia&ORLACCHIO,%20Rosa&HURTIER,%20Annabelle&DE%20GANNES,%20Florence%20Poulletier&rft.genre=article


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