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Nucleoside Binding by a Surface Lipoprotein Governs Conjugative ICE Acquisition in Ruminant Mycoplasmas

hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorDERRICHE, M’hamed
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorNOUVEL, Laurent Xavier
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorFAUVET, Calvin
hal.structure.identifierInstitut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement [INRAE]
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorMACH, Núria
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorSIMON, Elisa
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorPOT, Gwendoline
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorROBERT, Hortensia
hal.structure.identifierInstitut de pharmacologie et de biologie structurale [IPBS]
dc.contributor.authorSTELLA, Alexandre
hal.structure.identifierDepartamento de Sanidad Animal, Facultad de Veterinaria, CEIR Campus Mare Nostrum (CMN), Universidad de Murcia, Murcia, Spain
dc.contributor.authorDE LA FE, Christian
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorMAILLARD, Renaud
hal.structure.identifierDepartament de Bioquímica i Biologia Molecular, Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Barcelona, Spain
dc.contributor.authorTORRES-PUIG, Sergi
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorARFI, Yonathan
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorCITTI, Christine
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorBARANOWSKI, Eric
dc.date.issued2025-09-22
dc.description.abstractEnIntegrative and conjugative elements (ICEs) are major mediators of horizontal gene transfer (HGT) in bacteria. However, the role of recipient cells in their acquisition has received little attention. Using the ruminant pathogens Mycoplasma agalactiae and Mycoplasma bovis as minimal models, we combined genome-wide transposon mutagenesis with high-throughput mating assays to identify recipient factors required for ICE acquisition. The surface lipoprotein P48 emerged as the primary determinant of ICE uptake in both species. Structural and functional analyses revealed that P48 is the substrate-binding component of an ABC transporter with nucleoside-binding capacity. A single point mutation that abolished nucleoside binding drastically reduced ICE acquisition, demonstrating that P48-mediated nucleoside recognition is essential for conjugative transfer. However, ICE uptake did not require nucleoside transport, as inactivation of the transporter permease blocked nucleoside analog toxicity but not ICE invasion. Loss of P48 also triggered transcriptional activation of vestigial ICE genes, suggesting that surface recognition affects the intracellular state of the recipient. Remarkably, ICE transmission from recipient-derived donors was unaffected by P48 loss, underscoring its acquisition-specific role. Together, these results reveal a previously unrecognized, surface-exposed recipient factor critical for efficient ICE transfer in mycoplasmas and identify nucleotide binding as a central function in conjugation. By demonstrating that recipient-encoded functions can directly control ICE dissemination, this work challenges the donor-centric paradigm of bacterial conjugation and suggests new strategies to restrict horizontal gene flow in pathogenic and synthetic mycoplasmas.
dc.description.sponsorshipApproche rationnelle d'un vaccin Mycoplasma bovis
dc.language.isoen
dc.title.enNucleoside Binding by a Surface Lipoprotein Governs Conjugative ICE Acquisition in Ruminant Mycoplasmas
dc.typeDocument de travail - Pré-publication
dc.identifier.doi10.1101/2025.09.22.677790
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie
hal.identifierhal-05279369
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-05279369v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2025-09-22&rft.au=DERRICHE,%20M%E2%80%99hamed&NOUVEL,%20Laurent%20Xavier&FAUVET,%20Calvin&MACH,%20N%C3%BAria&SIMON,%20Elisa&rft.genre=preprint


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