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Water-soluble dendrigrafts bearing saccharidic moieties: Elaboration and application to enzyme linked OligoSorbent assay (ELOSA) diagnostic tests

dc.rights.licenseopen
hal.structure.identifierLaboratoire de Chimie des polymères organiques [LCPO]
dc.contributor.authorBERNARD, Julien
hal.structure.identifierLaboratoire de Chimie des polymères organiques [LCPO]
dc.contributor.authorSCHAPPACHER, Michel
hal.structure.identifierLaboratoire de Chimie des polymères organiques [LCPO]
hal.structure.identifierTeam 1 LCPO : Polymerization Catalyses & Engineering
dc.contributor.authorDEFFIEUX, Alain
hal.structure.identifierLaboratory for Chemistry of Novel Materials
dc.contributor.authorVIVILLE, Philippe
hal.structure.identifierLaboratory for Chemistry of Novel Materials
dc.contributor.authorLAZZARONI, Roberto
hal.structure.identifierSystèmes Macromoléculaires et Physiopathologie Humaine [SMPH]
dc.contributor.authorCHARLES, Marie-Hélène
hal.structure.identifierSystèmes Macromoléculaires et Physiopathologie Humaine [SMPH]
dc.contributor.authorCHARREYRE, Marie-Therese
hal.structure.identifierSystèmes Macromoléculaires et Physiopathologie Humaine [SMPH]
dc.contributor.authorDELAIR, Thierry
dc.date.accessioned2020
dc.date.available2020
dc.date.created2006
dc.date.issued2006
dc.identifier.issn1043-1802
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/20760
dc.description.abstractEnThe synthesis of a series of water-soluble galactopyranose-functionalized polystyrene-polyvinyl ether dendrigrafts and their characterization (in solution and thin solid deposits) have been achieved. The presence of external galactopyranose groups on dendritic polymers has been exploited to prepare dendrigraft-oligonucleotide conjugates using a simple one-step coupling procedure with amino-ended oligonucleotides (ODNs). Several parameters such as the peripherical density of hydrophilic branches, the polymerization degree of polystyrene or poly(hydroxyethyl vinyl ether) blocks, and the number of galactopyranose groups were tuned. A capture test with short labeled complementary ODNs (25 bases) confirmed the presence of covalently bound ODNs on various kinds of dendrigrafts. The ability of the dendritic polymers to enhance the sensitivity of enzyme-linked oligosorbent assay (ELOSA) diagnostic tests (detection of hepatitis B virus, DNA target of 2400 bases) was then evaluated, especially the influence of the macromolecular architecture and the impact of the structural parameters. The dendrigraft-ODN conjugate with the lower saccharide external density was found to lead to a very significant amplification of the fluorescence signal, corresponding to a limit of sensitivity of 10(9) DNA copies per milliliter (instead of 10(11) DNA copies per milliliter without using dendrigrafts). Conversely, the dendrigrafts exhibiting a very high number of branches and galactopyranose groups at their periphery were not able to induce a better sensitivity due to steric hindrance generated by the peripheral congestion on these polymers.
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.title.enWater-soluble dendrigrafts bearing saccharidic moieties: Elaboration and application to enzyme linked OligoSorbent assay (ELOSA) diagnostic tests
dc.typeArticle de revue
dc.identifier.doi10.1021/bc049708j
dc.subject.halChimie/Polymères
bordeaux.journalBioconjugate Chemistry
bordeaux.page6-14
bordeaux.volume17
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue19
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-00342098
hal.version1
dc.subject.itsaccharidic functions
dc.subject.itdiagnostic functionalization
dc.subject.itoligonucleotides
dc.subject.itimmobilization
dc.subject.itdendrigrafts
dc.subject.itnucleic acid probes
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00342098v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Bioconjugate%20Chemistry&rft.date=2006&rft.volume=17&rft.issue=19&rft.spage=6-14&rft.epage=6-14&rft.eissn=1043-1802&rft.issn=1043-1802&rft.au=BERNARD,%20Julien&SCHAPPACHER,%20Michel&DEFFIEUX,%20Alain&VIVILLE,%20Philippe&LAZZARONI,%20Roberto&rft.genre=article


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