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Monitoring free plasma hemoglobin in ECMO patients: a two-center comparative study of second-derivative spectrophotometry and hemolysis indexes.

dc.rights.licenseopenen_US
dc.contributor.authorSHARAIHA, Théo
dc.contributor.authorPELLECUIER, Nolan
hal.structure.identifierBoRdeaux Institute in onCology [Inserm U1312 - BRIC]
dc.contributor.authorDABERNAT, Sandrine
dc.contributor.authorMESTARI, Fouzi
dc.contributor.authorCOLOMBIÈS, Brigitte
hal.structure.identifierBoRdeaux Institute in onCology [Inserm U1312 - BRIC]
dc.contributor.authorRICHARD, Emmanuel
dc.contributor.authorALKOURI, Rana
dc.contributor.authorBONNEFONT-ROUSSELOT, Dominique
dc.contributor.authorRUCHETON, Benoit
hal.structure.identifierBiologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases
dc.contributor.authorBATS, Marie-Lise
dc.date.accessioned2025-09-01T07:46:30Z
dc.date.available2025-09-01T07:46:30Z
dc.date.issued2025-07-27
dc.identifier.issn1873-2933en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/207538
dc.description.abstractEnExtracorporeal membrane oxygenation (ECMO) can induce massive intravascular hemolysis, commonly quantified by plasma free hemoglobin (fHb) measurement. While manual spectrophotometry using second derivative spectrophotometry (sDS) is the reference method, a recent assessment tool based on the hemolysis index (HI) was developed for fHb determination on different automated systems. This study compares the performance of two HI methods-on Roche Cobas c502 and Abbott Architect c16000-versus sDS in 83 ECMO patients. Method validation was performed on both automated HI systems, including determination of linearity, precision, and analytical interference from lipemia and icterus. Plasma fHb measurements in 83 ECMO patients from two hospital centers were compared between the two HI-derived methods and two manual sDS methods (at 415 nm and 578 nm). Both HI-automated methods showed excellent linearity (0.04-20 g/L) and reproducibility (CVs between 2.3 % and 6.3 %). However, they exhibited sensitivity to interference by high lipemic index, leading to underestimation of fHb for Abbott Architect and overestimation for Roche Cobas. In non-lipemic ECMO samples with fHb levels >0.10 g/L, a very good correlation was found between HI and sDS, especially at 578 nm. However, discrepancies were observed in lipemic samples, leading to clinically relevant biases for fHb values >0.50 g/L. The Roche Cobas and Abbott Architect HI methods are reliable tools for monitoring fHb in ECMO patients, with good reproducibility and linearity. However, in the presence of significant lipemia, confirmation by sDS is recommended to ensure accurate assessment of hemolysis. This approach facilitates an efficient and automated monitoring of fHb, essential for daily managing ECMO-related complications and improving patient outcomes.
dc.language.isoENen_US
dc.title.enMonitoring free plasma hemoglobin in ECMO patients: a two-center comparative study of second-derivative spectrophotometry and hemolysis indexes.
dc.title.alternativeClin Biochemen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.clinbiochem.2025.110983en_US
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologieen_US
dc.identifier.pubmed40730295en_US
bordeaux.journalClinical Biochemistryen_US
bordeaux.page110983en_US
bordeaux.volume139en_US
bordeaux.hal.laboratoriesBiologie des maladies cardiovasculaires (BMC) - UMR 1034en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.identifierhal-05232391
hal.version1
hal.date.transferred2025-09-01T07:46:33Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcepubmed
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Clinical%20Biochemistry&rft.date=2025-07-27&rft.volume=139&rft.spage=110983&rft.epage=110983&rft.eissn=1873-2933&rft.issn=1873-2933&rft.au=SHARAIHA,%20Th%C3%A9o&PELLECUIER,%20Nolan&DABERNAT,%20Sandrine&MESTARI,%20Fouzi&COLOMBI%C3%88S,%20Brigitte&rft.genre=article


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