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Interaction with eukaryotic cells enhances the circularization and transcription of integrative and conjugative elements in Mycoplasma hominis.

hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBOUREIMA ABDOU, Bachir
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorSILVANT, Alicia
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorLE ROY, Chloé
dc.contributor.authorGUIRAUD, Jennifer
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBIENTZ, Léa
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBÉVEN, Laure
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorDUBOIS, Véronique
dc.contributor.authorBARANOWSKI, Eric
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBÉBÉAR, Cécile
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorPEREYRE, Sabine
dc.date.accessioned2025-07-23T02:05:39Z
dc.date.available2025-07-23T02:05:39Z
dc.date.issued2025
dc.identifier.issn1759-8753
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/207376
dc.description.abstractEnMobile genetic elements drive bacterial evolution by promoting genetic plasticity and adaptation; among them, mycoplasma Integrative and Conjugative Elements (ICEs) challenge the notion that mycoplasmas evolved solely through genome reduction. However, they remain poorly characterized, particularly in Mycoplasma hominis, which is a human genital pathogen. In this study, we investigated the circularization, transcription, and protein expression of M. hominis 4788 ICE (ICEHo4788) under the following four environmental conditions: axenic growth, coculture with HeLa cells, mitomycin C exposure, and thermal stress. During axenic growth, ICEHo4788 circularization peaked at 12 h with a 9.4-fold increase in the number of circular forms. Mitomycin C and cold shock induced only a moderate increase in circularization (approximately 3-fold), whereas heat shock significantly reduced the number of circular forms. In coculture with HeLa cells, a strong increase in the number of circular forms was observed at 72 h and 7 days postinfection with 10- and 23-fold increases, respectively. Coculture also led to a 5- to 23-fold increase in transcription at 7 days, whereas axenic growth, mitomycin C exposure, and thermal stress did not differ. Proteomic analysis revealed that MhoH(a), CDS18, CDS17, and CDS11 were significantly overexpressed at 12 h. Neither mitomycin C nor cold shock induced changes in ICE-related proteins, but heat shock upregulated two M. hominis molecular chaperones and ICEHo4788 MhoH(a). In conclusion, environmental conditions modulate M. hominis ICE activity with strong stimulation in the presence of eukaryotic cells. These findings offer new insights into the regulation and environmental responses of M. hominis ICEs.
dc.language.isoen
dc.publisherBioMed Central
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enMycoplasma hominis
dc.subject.enCircularization
dc.subject.enHeLa cells
dc.subject.enIntegrative and conjugative element
dc.subject.enProtein expression
dc.subject.enTranscription
dc.title.enInteraction with eukaryotic cells enhances the circularization and transcription of integrative and conjugative elements in Mycoplasma hominis.
dc.typeArticle de revue
dc.identifier.doi10.1186/s13100-025-00364-x
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
dc.subject.halSciences du Vivant [q-bio]/Biodiversité/Evolution [q-bio.PE]
bordeaux.journalMobile DNA
bordeaux.page26
bordeaux.volume16
bordeaux.hal.laboratoriesBiologie du Fruit & Pathologie (BFP) - UMR 1332*
bordeaux.issue1
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-05167347
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-05167347v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Mobile%20DNA&rft.date=2025&rft.volume=16&rft.issue=1&rft.spage=26&rft.epage=26&rft.eissn=1759-8753&rft.issn=1759-8753&rft.au=BOUREIMA%20ABDOU,%20Bachir&SILVANT,%20Alicia&LE%20ROY,%20Chlo%C3%A9&GUIRAUD,%20Jennifer&BIENTZ,%20L%C3%A9a&rft.genre=article


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