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Statistical method inference cMFA for multi-omics data integration in microbial community models

hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
hal.structure.identifierPleiade, from patterns to models in computational biodiversity and biotechnology [PLEIADE]
dc.contributor.authorTATHO DJEANOU, Sthyve Junior
hal.structure.identifierPleiade, from patterns to models in computational biodiversity and biotechnology [PLEIADE]
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLABARTHE, Simon
hal.structure.identifierInstitut Sophia Agrobiotech [ISA]
hal.structure.identifierModélisation et commande de systèmes biologiques et écologiques [MACBES]
hal.structure.identifierUniversité Côte d'Azur [UniCA]
dc.contributor.authorBALDAZZI, Valentina
dc.date.accessioned2025-07-16T02:01:28Z
dc.date.available2025-07-16T02:01:28Z
dc.date.conference2025-07-08
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/207332
dc.description.abstractEnUnderstanding microbial community functions is challenging due to complex interactions and assembly mechanisms; however, advances in sequencing have enabled the collection of multi-omics data, including population counts and metabolomic or metatranscriptomic data. Our main objective is to develop a mathematical model capable of integrating time series of multiomics data at a community scale.We introduce the community metabolic flux analysis (cMFA) method, which generalizes metabolic flux analyses,, using a list of time series data of experimentally measured production and consumption rates of metabolites and microorganism growth. We aim to infer, for each member of the microbial community, the intracellular distribution of metabolic fluxes. This is a high-dimensional constrained linear regression problem, informed by mass conservation constraints and metatranscriptomic data, encoded in the penalty term. The difficulty here is in accurately inferring latent internal rates from a few observations of exchange fluxes.We evaluated the cMFA method on synthetic data from dynamic models of increasingly complex microbial communities, based on metabolic models of different mutants of Escherichia coli using dynamic flux balance analysis (dFBA). Synthetic metatranscriptomic data were obtained from internal metabolic fluxes in the dynamic model. Different regularization terms were tested, including different levels of sparsity, for the selected penalty weight . To evaluate the robustness of the method, multiple benchmarks were tested. These included assessments of the robustness of the method to data noise, incomplete meta-transcriptomic data, and inaccurate prior knowledge of metabolic import rates. Currently, we are working with real data and expanding the study to a larger microbial community.
dc.description.sponsorshipComputationel models of crop plant microbial biodiversity - ANR-22-PEAE-0011
dc.language.isoen
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enDynamical system
dc.subject.enApplied mathematics
dc.subject.enInference
dc.subject.enBiological system
dc.title.enStatistical method inference cMFA for multi-omics data integration in microbial community models
dc.title.enBenchmark of the method on data noise, incomplete meta-transcriptomic data, and inaccurate prior knowledge of metabolic import rates
dc.typeAutre communication scientifique (congrès sans actes - poster - séminaire...)
dc.subject.halMathématiques [math]
dc.subject.halSciences du Vivant [q-bio]/Bio-Informatique, Biologie Systémique [q-bio.QM]
bordeaux.hal.laboratoriesBioGeCo (Biodiversité Gènes & Communautés) - UMR 1202*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionINRAE
bordeaux.conference.titleJOBIM 2025 - Journées Ouvertes en Biologie , Informatique et mathématiques
bordeaux.countryFR
bordeaux.conference.cityBordeaux
bordeaux.peerReviewedoui
hal.identifierhal-05163368
hal.version1
hal.invitednon
hal.proceedingsnon
hal.conference.organizerSociété Française de BioInformatique (SFBI)
hal.conference.end2025-07-11
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-05163368v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=TATHO%20DJEANOU,%20Sthyve%20Junior&LABARTHE,%20Simon&BALDAZZI,%20Valentina&rft.genre=conference


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