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Detection of hibiscus chlorotic ringspot virus, citrus exocortis viroid and citrus viroid VI in China rose from Italy using high-throughput sequencing

dc.contributor.authorPARRELLA, Giuseppe
dc.contributor.authorFAURE, Chantal
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMARAIS, Armelle
dc.contributor.authorTROIANO, Elisa
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
dc.date.accessioned2025-06-24T02:10:30Z
dc.date.available2025-06-24T02:10:30Z
dc.date.issued2024-09-16
dc.identifier.issn0031-9465
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/207101
dc.description.abstractEnIn Spring 2024, a symptomatic plant of China rose (Hibiscus rosa-sinensis) showing leaf yellowing and deformation, located in the province of Naples (South Italy), was re-sampled for further investigations. Total RNAs were purified from leaves and subjected to Illumina HTS analysis. Results confirmed that the plant was infected by hibiscus chlorotic ringspot virus (HCRSV), citrus exocortis viroid (CEVd), and citrus viroid VI (CVd-VI). The two viroids had not been previously detected in China rose. HTS results were confirmed by RT-PCR in the re-sampled plant, and in three nearby China rose plants with the same symptoms, using specific primer pairs for the three pathogens, followed by Sanger sequencing and BLAST analysis of the sequences. Different results obtained can be due to differing sensitivity and specificity in HTS-based detection of plant viruses. This is the first report of multiple infections by HCRS, CEVd and CVd-VI in China rose, which is also a new host for both of the viroids.
dc.language.isoen
dc.publisherFirenze University Press
dc.title.enDetection of hibiscus chlorotic ringspot virus, citrus exocortis viroid and citrus viroid VI in China rose from Italy using high-throughput sequencing
dc.typeArticle de revue
dc.identifier.doi10.36253/phyto-15435
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalPhytopathologia Mediterranea
bordeaux.page255-257
bordeaux.volume63
bordeaux.hal.laboratoriesBiologie du Fruit & Pathologie (BFP) - UMR 1332*
bordeaux.issue2
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-05125730
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-05125730v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Phytopathologia%20Mediterranea&rft.date=2024-09-16&rft.volume=63&rft.issue=2&rft.spage=255-257&rft.epage=255-257&rft.eissn=0031-9465&rft.issn=0031-9465&rft.au=PARRELLA,%20Giuseppe&FAURE,%20Chantal&MARAIS,%20Armelle&TROIANO,%20Elisa&CANDRESSE,%20Thierry&rft.genre=article


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