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Modulating the activity of the APC /C regulator SISAMBA improves the sugar and antioxidant content of tomato fruits

hal.structure.identifierUniversidade de São Paulo = University of São Paulo [USP]
dc.contributor.authorDE OLIVEIRA, Perla Novais
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
dc.contributor.authorDE SOUZA, Leonardo Perez
hal.structure.identifierUniversidade de São Paulo = University of São Paulo [USP]
dc.contributor.authorFERREIRA, Pedro Boscariol
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUXION, Jean Phillippe
hal.structure.identifierUniversidade de São Paulo = University of São Paulo [USP]
dc.contributor.authorDA SILVA, Luis Felipe Correa
hal.structure.identifierUniversidade de São Paulo = University of São Paulo [USP]
dc.contributor.authorCHANG, Ana Isabela
hal.structure.identifierUniversidade de São Paulo = University of São Paulo [USP]
dc.contributor.authorSALEME, Marina De Lyra Soriano
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
hal.structure.identifierHuazhong Agricultural University [Wuhan] [HZAU]
dc.contributor.authorZHU, Feng
hal.structure.identifierUniversiteit Gent = Ghent University = Université de Gand [UGENT]
dc.contributor.authorGARCÍA, Sara Selma
hal.structure.identifierUniversiteit Gent = Ghent University = Université de Gand [UGENT]
dc.contributor.authorDE BEUKELAER, Herman
hal.structure.identifierIn Press Consultoria e Comunicação Científica
dc.contributor.authorMARTINS, Marina
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
dc.contributor.authorROSADO-SOUZA, Laise
hal.structure.identifierUniversidade de São Paulo = University of São Paulo [USP]
dc.contributor.authorPINO, Lilian Ellen
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBOLLIER, Norbert
hal.structure.identifierUniversidade de São Paulo = University of São Paulo [USP]
dc.contributor.authorPERES, Lázaro Eustáquio Pereira
hal.structure.identifierUniversiteit Gent = Ghent University = Université de Gand [UGENT]
dc.contributor.authorVANDEPOELE, Klaas
hal.structure.identifierUniversiteit Gent = Ghent University = Université de Gand [UGENT]
hal.structure.identifierStellenbosch University
dc.contributor.authorGOOSSENS, Alain
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGONZALEZ, Nathalie
hal.structure.identifierMax Planck Institute of Molecular Plant Physiology [MPI-MP]
dc.contributor.authorFERNIE, Alisdair
hal.structure.identifierUniversidade de São Paulo = University of São Paulo [USP]
dc.contributor.authorELOY, Nubia Barbosa
dc.date.accessioned2025-06-20T02:07:41Z
dc.date.available2025-06-20T02:07:41Z
dc.date.issued2025-06-08
dc.identifier.issn1467-7644
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/206969
dc.description.abstractEnThe Anaphase-Promoting Complex/Cyclosome (APC/C) is an E3 ubiquitin ligase that plays a crucial role in ubiquitin-dependent proteolysis of key cell cycle regulators, which is completed by the 26S proteasome. Previously, SAMBA, a plant-specific regulator of the APC/C, was identified in Arabidopsis as a critical factor controlling organ size through the regulation of cell proliferation. Here, by assessing its role in the crop tomato (Solanum lycopersicum), we confirm that SAMBA is a conserved APC/C regulator in plants and shows additional roles, including the modulation of fruit shape and changes in sugar metabolism. Two slsamba genome-edited lines were produced and characterized, and showed delayed growth, reduced plant size, and altered fruit morphology, which were linked to changes in cell division and expansion. Notably, untargeted metabolomics revealed altered flavonoid profiles, along with elevated Brix values in the fruits, indicating a sweeter taste. Accordingly, transcriptomics uncovered a change in temporal gene expression gradients during early fruit development, correlating with the alterations in sugar metabolism and revealing changes in cell wall biosynthesis genes. This study provides the first evidence of SAMBA's role in regulating fruit development, metabolic content, and ultimately, quality. These important findings offer potential applications for improving the nutritional quality and overall performance of tomatoes.
dc.language.isoen
dc.publisherWiley
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/
dc.subject.enAPC/C
dc.subject.enCRISPR/Ca9
dc.subject.enfruit development
dc.subject.enmetabolic changes
dc.subject.entomato
dc.subject.entranscriptomic analysis
dc.title.enModulating the activity of the APC /C regulator SISAMBA improves the sugar and antioxidant content of tomato fruits
dc.typeArticle de revue
dc.identifier.doi10.1111/pbi.70149
dc.subject.halSciences de l'environnement
bordeaux.journalPlant Biotechnology Journal
bordeaux.hal.laboratoriesBiologie du Fruit & Pathologie (BFP) - UMR 1332*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-05120282
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-05120282v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20Biotechnology%20Journal&rft.date=2025-06-08&rft.eissn=1467-7644&rft.issn=1467-7644&rft.au=DE%20OLIVEIRA,%20Perla%20Novais&DE%20SOUZA,%20Leonardo%20Perez&FERREIRA,%20Pedro%20Boscariol&MAUXION,%20Jean%20Phillippe&DA%20SILVA,%20Luis%20Felipe%20Correa&rft.genre=article


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