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Contrasting effect of clathrin or uk1_LRR gene silencing in the vector Euscelidius variegatus on its colonization by flavescence dorée phytoplasma

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorARRICAU-BOUVERY, Nathalie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDUBRANA-OURABAH, Marie-Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDURET, Sybille
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANUTO, Francesca
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFOISSAC, Xavier
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMALEMBIC-MAHER, Sylvie
dc.date.accessioned2025-06-06T02:06:11Z
dc.date.available2025-06-06T02:06:11Z
dc.date.conference2025-05-20
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/206827
dc.description.abstractEnThe flavescence dorée (FD) phytoplasma is a quarantine bacteria associated with a severe disease of grapevine and transmitted by the insect vector Scaphoideus titanus. In experimental conditions, FD phytoplasma is also transmitted by Euscelidius variegatus to broad bean in which it multiplies and also causes symptoms. To be transmitted to plants, phytoplasmas must invade different cell types of their insect vectors including midgut epithelial and salivary gland cells. The process of cellular endocytosis involves both bacterial and eucaryotic factors such as adhesins and receptors. FD phytoplasma expresses variable membrane (Vmp) proteins at its surface and the insect protein Uk1_LRR is one of the putative receptors of the adhesin VmpA (Arricau-Bouvery et al., 2021; Canuto et al., 2023). Once attached to insect cells, FD phytoplasma enters into eucaryotic cells using clathrin-mediated endocytosis (Arricau-Bouvery et al., 2023). In the present study, we have shown that VmpA-coated fluorescent beads enter E. variegatus cultured cells in a Uk1_LRR and clathrin dependent manner. In vivo experiments show that dsRNA mediated silencing of clathrin gene significantly reduced the colonization of E. variegatus by FD phytoplasma and the transmission to the plant Vicia faba. These results confirm the implication of clathrin in insect cells’ invasion by FD phytoplasma during E. variegatus colonization. Interestingly, silencing uk1_LRR gene increased the colonization of E. variegatus by FD phytoplasma without effect on the transmission to Vicia faba. We therefore hypothesize that uk1_LRR could be implicated in both FD phytoplasma recognition and immune response of E. variegatus toward FD phytoplasma entry into insect cells.
dc.language.isoen
dc.title.enContrasting effect of clathrin or uk1_LRR gene silencing in the vector Euscelidius variegatus on its colonization by flavescence dorée phytoplasma
dc.typeAutre communication scientifique (congrès sans actes - poster - séminaire...)
dc.subject.halSciences du Vivant [q-bio]
bordeaux.hal.laboratoriesBiologie du Fruit & Pathologie (BFP) - UMR 1332*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionINRAE
bordeaux.conference.title12e Colloque de la Société Française de Phytopathologie
bordeaux.countryFR
bordeaux.conference.cityBordeaux
bordeaux.peerReviewednon
hal.identifierhal-05095766
hal.version1
hal.invitednon
hal.proceedingsnon
hal.conference.end2025-05-23
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-05095766v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=ARRICAU-BOUVERY,%20Nathalie&DUBRANA-OURABAH,%20Marie-Pierre&DURET,%20Sybille&CANUTO,%20Francesca&FOISSAC,%20Xavier&rft.genre=conference


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