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Rubber Elongation Factor (REF), a Major Allergen Component in Hevea brasiliensis Latex Has Amyloid Properties

dc.rights.licenseopen
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
dc.contributor.authorBERTHELOT, Karine
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLECOMTE, Sophie
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
dc.contributor.authorESTEVEZ, Yannick
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorCOULARY-SALIN, Bénédicte
hal.structure.identifierCentre de recherches Paul Pascal [CRPP]
dc.contributor.authorBENTALEB, Ahmed
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorCULLIN, Christophe
ORCID: 0000-0003-4110-4677
IDREF: 85920959
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 1 LCPO : Polymerization Catalyses & Engineering
dc.contributor.authorDEFFIEUX, Alain
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 1 LCPO : Polymerization Catalyses & Engineering
dc.contributor.authorPERUCH, Frédéric
IDREF: 152900748
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2012
dc.identifier.issn1932-6203
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/20477
dc.description.abstractEnREF (Hevb1) and SRPP (Hevb3) are two major components of Hevea brasiliensis latex, well known for their allergenic properties. They are obviously taking part in the biosynthesis of natural rubber, but their exact function is still unclear. They could be involved in defense/stress mechanisms after tapping or directly acting on the isoprenoid biosynthetic pathway. The structure of these two proteins is still not described. In this work, it was discovered that REF has amyloid properties, contrary to SRPP. We investigated their structure by CD, TEM, ATR-FTIR and WAXS and neatly showed the presence of beta-sheet organized aggregates for REF, whereas SRPP mainly fold as a helical protein. Both proteins are highly hydrophobic but differ in their interaction with lipid monolayers used to mimic the monomembrane surrounding the rubber particles. Ellipsometry experiments showed that REF seems to penetrate deeply into the monolayer and SRPP only binds to the lipid surface. These results could therefore clarify the role of these two paralogous proteins in latex production, either in the coagulation of natural rubber or in stress-related responses. To our knowledge, this is the first report of an amyloid formed from a plant protein. This suggests also the presence of functional amyloid in the plant kingdom.
dc.language.isoen
dc.publisherPublic Library of Science
dc.subject.enNR latex
dc.subject.enrubber elongation factor
dc.subject.enhevea
dc.title.enRubber Elongation Factor (REF), a Major Allergen Component in Hevea brasiliensis Latex Has Amyloid Properties
dc.typeArticle de revue
dc.identifier.doi10.1371/journal.pone.0048065
dc.subject.halChimie/Polymères
bordeaux.journalPLoS ONE
bordeaux.pagee48065
bordeaux.volume7
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue10
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-00757298
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00757298v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS%20ONE&rft.date=2012&rft.volume=7&rft.issue=10&rft.spage=e48065&rft.epage=e48065&rft.eissn=1932-6203&rft.issn=1932-6203&rft.au=BERTHELOT,%20Karine&LECOMTE,%20Sophie&ESTEVEZ,%20Yannick&COULARY-SALIN,%20B%C3%A9n%C3%A9dicte&BENTALEB,%20Ahmed&rft.genre=article


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