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A yeast toxic mutant of HET-s amyloid disrupts membrane integrity

dc.rights.licenseopen
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorTA, Ha Phuong
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 1 LCPO : Polymerization Catalyses & Engineering
dc.contributor.authorBERTHELOT, Karine
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorCOULARY-SALIN, Bénédicte
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCASTANO, Sabine
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorDESBAT, Bernard
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBONNAFOUS, Pierre
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLAMBERT, Olivier
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorALVES, Isabel
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorCULLIN, Christophe
ORCID: 0000-0003-4110-4677
IDREF: 85920959
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLECOMTE, Sophie
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2012
dc.identifier.issn0005-2736
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/20454
dc.description.abstractEnMany studies have pointed out the interaction between amyloids and membranes, and their potential involvement in amyloid toxicity. Previously, we generated a yeast toxic amyloid mutant (M8) from the harmless amyloid protein by changing a few residues of the Prion Forming Domain of HET-s (PFD HET-s(218-289)) and clearly demonstrated the complete different behaviors of the non-toxic Wild Type (WT) and toxic amyloid (called M8) in terms of fiber morphology, aggregation kinetics and secondary structure. In this study, we compared the interaction of both proteins (WI and M8) with membrane models, as liposomes or supported bilayers. We first demonstrated that the toxic protein (M8) induces a significant leakage of liposomes formed with negatively charged lipids and promotes the formation of microdomains inside the lipid bilayer (as potential "amyloid raft"), whereas the non-toxic amyloid (WT) only binds to the membrane without further perturbations. The secondary structure of both amyloids interacting with membrane is preserved, but the antisymmetric PO2- vibration is strongly shifted in the presence of M8. Secondly, we established that the presence of membrane models catalyzes the amyloidogenesis of both proteins. Cryo-TEM (cryo-transmission electron microscopy) images show the formation of long HET-s fibers attached to liposomes, whereas a large aggregation of the toxic M8 seems to promote a membrane disruption. This study allows us to conclude that the toxicity of the M8 mutant could be due to its high propensity to interact and disrupt lipid membranes
dc.language.isoen
dc.publisherElsevier
dc.subject.enAmyloid toxicity
dc.subject.enHET-s
dc.subject.enPWR
dc.subject.enATR-FTIR
dc.subject.enLeakage
dc.subject.enMembrane
dc.title.enA yeast toxic mutant of HET-s amyloid disrupts membrane integrity
dc.typeArticle de revue
dc.identifier.doi10.1016/j.bbamem.2012.04.013
dc.subject.halChimie/Polymères
bordeaux.journalBiochimica et Biophysica Acta:Biomembranes
bordeaux.page2325-2334
bordeaux.volume1818
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue9
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-00803773
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00803773v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biochimica%20et%20Biophysica%20Acta:Biomembranes&rft.date=2012&rft.volume=1818&rft.issue=9&rft.spage=2325-2334&rft.epage=2325-2334&rft.eissn=0005-2736&rft.issn=0005-2736&rft.au=TA,%20Ha%20Phuong&BERTHELOT,%20Karine&COULARY-SALIN,%20B%C3%A9n%C3%A9dicte&CASTANO,%20Sabine&DESBAT,%20Bernard&rft.genre=article


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